The Human Skeletal Muscle Proteome Project: a reappraisal of the current literature

Marta Gonzalez-Freire, Richard D. Semba, Ceereena Ubaida-Mohien, Elisa Fabbri, Paul Scalzo, Kurt Højlund, Craig Dufresne, Alexey Lyashkov, Luigi Ferrucci

Research output: Contribution to journalReview articlepeer-review

Abstract

Skeletal muscle is a large organ that accounts for up to half the total mass of the human body. A progressive decline in muscle mass and strength occurs with ageing and in some individuals configures the syndrome of ‘sarcopenia’, a condition that impairs mobility, challenges autonomy, and is a risk factor for mortality. The mechanisms leading to sarcopenia as well as myopathies are still little understood. The Human Skeletal Muscle Proteome Project was initiated with the aim to characterize muscle proteins and how they change with ageing and disease. We conducted an extensive review of the literature and analysed publically available protein databases. A systematic search of peer-reviewed studies was performed using PubMed. Search terms included ‘human’, ‘skeletal muscle’, ‘proteome’, ‘proteomic(s)’, and ‘mass spectrometry’, ‘liquid chromatography-mass spectrometry (LC-MS/MS)’. A catalogue of 5431 non-redundant muscle proteins identified by mass spectrometry-based proteomics from 38 peer-reviewed scientific publications from 2002 to November 2015 was created. We also developed a nosology system for the classification of muscle proteins based on localization and function. Such inventory of proteins should serve as a useful background reference for future research on changes in muscle proteome assessed by quantitative mass spectrometry-based proteomic approaches that occur with ageing and diseases. This classification and compilation of the human skeletal muscle proteome can be used for the identification and quantification of proteins in skeletal muscle to discover new mechanisms for sarcopenia and specific muscle diseases that can be targeted for the prevention and treatment.

Original languageEnglish (US)
Pages (from-to)5-18
Number of pages14
JournalJournal of Cachexia, Sarcopenia and Muscle
Volume8
Issue number1
DOIs
StatePublished - Feb 1 2017

Keywords

  • Ageing
  • Mass spectrometry
  • Post-translational modifications
  • Proteomics
  • Skeletal muscle

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physiology (medical)

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