The human Jurkat (FHCRC-11) cell line is heterogeneous in ploidy and cell size and releases detergent-soluble DNA

K. A. LaGree, A. T. Lee, G. Stetten, P. R. Strauss

Research output: Contribution to journalArticle

Abstract

Jurkat (FHCRC-11) cells, a human lymphoblastic leukemic line, were characterized as being hypotetraploid with a characteristic deletion in the short arm of chromosome 2 from the terminus to band 24. Although Jurkat cells were size heterogeneous, variability in ploidy was not correlated with density and size differences observed when cells were fractionated by means of gradient centrifugation using Ny-codenz as the supporting medium. Also no difference was seen in the chromosome distribution of cells cultured from different portions of the gradient. During cel division Jurkat cells incorporated [3H]thymidine ([3H]TdR) into newly made DNA, including a small percentage that was released into the soluble fraction upon detergent lysis. Small light cells from the top portion of the gradient were more efficient on a per cell basis in incorporating [3H]TdR into DNA from both the detergent-soluble and detergent-insoluble fractions. However, due to the hypotetraploid nature of these cells a definitive assignment to a specific stage in the cell cycle was not possible.

Original languageEnglish (US)
Pages (from-to)686-690
Number of pages5
JournalExperimental Hematology
Volume16
Issue number8
StatePublished - 1988
Externally publishedYes

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Ploidies
Cell Size
Detergents
Cell Line
DNA
Jurkat Cells
Chromosomes, Human, Pair 2
Centrifugation
Thymidine
Cultured Cells
Cell Cycle
Chromosomes

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

The human Jurkat (FHCRC-11) cell line is heterogeneous in ploidy and cell size and releases detergent-soluble DNA. / LaGree, K. A.; Lee, A. T.; Stetten, G.; Strauss, P. R.

In: Experimental Hematology, Vol. 16, No. 8, 1988, p. 686-690.

Research output: Contribution to journalArticle

LaGree, K. A. ; Lee, A. T. ; Stetten, G. ; Strauss, P. R. / The human Jurkat (FHCRC-11) cell line is heterogeneous in ploidy and cell size and releases detergent-soluble DNA. In: Experimental Hematology. 1988 ; Vol. 16, No. 8. pp. 686-690.
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