The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor η Chain

Marco A. Purbhoo, Jonathan M. Boulter, David A. Price, Anne Lise Vuidepot, Christopher Hourigan, P. Rod Dunbar, Kara Olson, Sara J. Dawson, Rodney E. Phillips, Bent K. Jakobsen, John I. Bell, Andrew K. Sewell

Research output: Contribution to journalArticle

Abstract

Recognition of antigen by cytotoxic T lymphocytes (CTL) is determined by interaction of both the T cell receptor and its CD8 coreceptor with peptide-major histocompatibility complex (pMHC) class I molecules. We examine the relative roles of these receptors in the activation of human CTL using mutations in MHC class I designed to diminish or abrogate the CD8/pMHC interaction. We use surface plasmon resonance to determine that point mutation of the α3 loop of HLA A2 abrogates the CD8/pMHC interaction without affecting the affinity of the T cell receptor/pMHC interaction. Antigen-presenting cells expressing HLA A2 which does not bind to CD8 fail to activate CTL at any peptide concentration. Comparison of CTL activation by targets expressing HLA A2 with normal, abrogated, or diminished CD8/pMHC interaction show that the CD8/pMHC interaction enhances sensitivity to antigen. We determine that the biochemical basis for coreceptor dependence is the activation of the 23-kDa phosphoform of the CD3η chain. In addition, we produce mutant MHC class I multimers that specifically stain but do not activate CTL. These reagents may prove useful in circumventing undesirable activation-related perturbation of intracellular processes when pMHC multimers are used to phenotype antigen-specific CD8+ lymphocytes.

Original languageEnglish (US)
Pages (from-to)32786-32792
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number35
DOIs
StatePublished - Aug 31 2001
Externally publishedYes

Fingerprint

CD27 Antigens
Phosphorylation
T-Cell Antigen Receptor
Major Histocompatibility Complex
T-cells
Cytotoxic T-Lymphocytes
Peptides
HLA-A2 Antigen
Chemical activation
Antigens
CD8 Antigens
Lymphocytes
Surface Plasmon Resonance
Surface plasmon resonance
Antigen-Presenting Cells
Lymphocyte Activation
Point Mutation
Coloring Agents
Phenotype
Mutation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor η Chain. / Purbhoo, Marco A.; Boulter, Jonathan M.; Price, David A.; Vuidepot, Anne Lise; Hourigan, Christopher; Rod Dunbar, P.; Olson, Kara; Dawson, Sara J.; Phillips, Rodney E.; Jakobsen, Bent K.; Bell, John I.; Sewell, Andrew K.

In: Journal of Biological Chemistry, Vol. 276, No. 35, 31.08.2001, p. 32786-32792.

Research output: Contribution to journalArticle

Purbhoo, MA, Boulter, JM, Price, DA, Vuidepot, AL, Hourigan, C, Rod Dunbar, P, Olson, K, Dawson, SJ, Phillips, RE, Jakobsen, BK, Bell, JI & Sewell, AK 2001, 'The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor η Chain', Journal of Biological Chemistry, vol. 276, no. 35, pp. 32786-32792. https://doi.org/10.1074/jbc.M102498200
Purbhoo, Marco A. ; Boulter, Jonathan M. ; Price, David A. ; Vuidepot, Anne Lise ; Hourigan, Christopher ; Rod Dunbar, P. ; Olson, Kara ; Dawson, Sara J. ; Phillips, Rodney E. ; Jakobsen, Bent K. ; Bell, John I. ; Sewell, Andrew K. / The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor η Chain. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 35. pp. 32786-32792.
@article{9ec3936997024a10aae111cb5b65f994,
title = "The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor η Chain",
abstract = "Recognition of antigen by cytotoxic T lymphocytes (CTL) is determined by interaction of both the T cell receptor and its CD8 coreceptor with peptide-major histocompatibility complex (pMHC) class I molecules. We examine the relative roles of these receptors in the activation of human CTL using mutations in MHC class I designed to diminish or abrogate the CD8/pMHC interaction. We use surface plasmon resonance to determine that point mutation of the α3 loop of HLA A2 abrogates the CD8/pMHC interaction without affecting the affinity of the T cell receptor/pMHC interaction. Antigen-presenting cells expressing HLA A2 which does not bind to CD8 fail to activate CTL at any peptide concentration. Comparison of CTL activation by targets expressing HLA A2 with normal, abrogated, or diminished CD8/pMHC interaction show that the CD8/pMHC interaction enhances sensitivity to antigen. We determine that the biochemical basis for coreceptor dependence is the activation of the 23-kDa phosphoform of the CD3η chain. In addition, we produce mutant MHC class I multimers that specifically stain but do not activate CTL. These reagents may prove useful in circumventing undesirable activation-related perturbation of intracellular processes when pMHC multimers are used to phenotype antigen-specific CD8+ lymphocytes.",
author = "Purbhoo, {Marco A.} and Boulter, {Jonathan M.} and Price, {David A.} and Vuidepot, {Anne Lise} and Christopher Hourigan and {Rod Dunbar}, P. and Kara Olson and Dawson, {Sara J.} and Phillips, {Rodney E.} and Jakobsen, {Bent K.} and Bell, {John I.} and Sewell, {Andrew K.}",
year = "2001",
month = "8",
day = "31",
doi = "10.1074/jbc.M102498200",
language = "English (US)",
volume = "276",
pages = "32786--32792",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "35",

}

TY - JOUR

T1 - The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor η Chain

AU - Purbhoo, Marco A.

AU - Boulter, Jonathan M.

AU - Price, David A.

AU - Vuidepot, Anne Lise

AU - Hourigan, Christopher

AU - Rod Dunbar, P.

AU - Olson, Kara

AU - Dawson, Sara J.

AU - Phillips, Rodney E.

AU - Jakobsen, Bent K.

AU - Bell, John I.

AU - Sewell, Andrew K.

PY - 2001/8/31

Y1 - 2001/8/31

N2 - Recognition of antigen by cytotoxic T lymphocytes (CTL) is determined by interaction of both the T cell receptor and its CD8 coreceptor with peptide-major histocompatibility complex (pMHC) class I molecules. We examine the relative roles of these receptors in the activation of human CTL using mutations in MHC class I designed to diminish or abrogate the CD8/pMHC interaction. We use surface plasmon resonance to determine that point mutation of the α3 loop of HLA A2 abrogates the CD8/pMHC interaction without affecting the affinity of the T cell receptor/pMHC interaction. Antigen-presenting cells expressing HLA A2 which does not bind to CD8 fail to activate CTL at any peptide concentration. Comparison of CTL activation by targets expressing HLA A2 with normal, abrogated, or diminished CD8/pMHC interaction show that the CD8/pMHC interaction enhances sensitivity to antigen. We determine that the biochemical basis for coreceptor dependence is the activation of the 23-kDa phosphoform of the CD3η chain. In addition, we produce mutant MHC class I multimers that specifically stain but do not activate CTL. These reagents may prove useful in circumventing undesirable activation-related perturbation of intracellular processes when pMHC multimers are used to phenotype antigen-specific CD8+ lymphocytes.

AB - Recognition of antigen by cytotoxic T lymphocytes (CTL) is determined by interaction of both the T cell receptor and its CD8 coreceptor with peptide-major histocompatibility complex (pMHC) class I molecules. We examine the relative roles of these receptors in the activation of human CTL using mutations in MHC class I designed to diminish or abrogate the CD8/pMHC interaction. We use surface plasmon resonance to determine that point mutation of the α3 loop of HLA A2 abrogates the CD8/pMHC interaction without affecting the affinity of the T cell receptor/pMHC interaction. Antigen-presenting cells expressing HLA A2 which does not bind to CD8 fail to activate CTL at any peptide concentration. Comparison of CTL activation by targets expressing HLA A2 with normal, abrogated, or diminished CD8/pMHC interaction show that the CD8/pMHC interaction enhances sensitivity to antigen. We determine that the biochemical basis for coreceptor dependence is the activation of the 23-kDa phosphoform of the CD3η chain. In addition, we produce mutant MHC class I multimers that specifically stain but do not activate CTL. These reagents may prove useful in circumventing undesirable activation-related perturbation of intracellular processes when pMHC multimers are used to phenotype antigen-specific CD8+ lymphocytes.

UR - http://www.scopus.com/inward/record.url?scp=0035980059&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035980059&partnerID=8YFLogxK

U2 - 10.1074/jbc.M102498200

DO - 10.1074/jbc.M102498200

M3 - Article

C2 - 11438524

AN - SCOPUS:0035980059

VL - 276

SP - 32786

EP - 32792

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 35

ER -