TY - JOUR
T1 - The human carotid body transcriptome with focus on oxygen sensing and inflammation - a comparative analysis
AU - Mkrtchian, Souren
AU - Kåhlin, Jessica
AU - Ebberyd, Anette
AU - Gonzalez, Constancio
AU - Sanchez, Diego
AU - Balbir, Alexander
AU - Kostuk, Eric W.
AU - Shirahata, Machiko
AU - Fagerlund, Malin Jonsson
AU - Eriksson, Lars I.
N1 - Funding Information:
This work, supported by European Community under the contract of Association between EURATOM/CEA, was carried out within the framework of the European Fusion Development Agreement (EFDA).
PY - 2012/8
Y1 - 2012/8
N2 - The carotid body (CB) is the key oxygen sensing organ. While the expression of CB specific genes is relatively well studied in animals, corresponding data for the human CB are missing. In this study we used five surgically removed human CBs to characterize the CB transcriptome with microarray and PCR analyses, and compared the results with mice data. In silico approaches demonstrated a unique gene expression profile of the human and mouse CB transcriptomes and an unexpected upregulation of both human and mouse CB genes involved in the inflammatory response compared to brain and adrenal gland data. Human CBs express most of the genes previously proposed to be involved in oxygen sensing and signalling based on animal studies, including NOX2, AMPK, CSE and oxygen sensitive K+ channels. In the TASK subfamily of K+ channels, TASK-1 is expressed in human CBs, while TASK-3 and TASK-5 are absent, although we demonstrated both TASK-1 and TASK-3 in one of the mouse reference strains. Maxi-K was expressed exclusively as the spliced variant ZERO in the human CB. In summary, the human CB transcriptome shares important features with the mouse CB, but also differs significantly in the expression of a number of CB chemosensory genes. This study provides key information for future functional investigations on the human carotid body.
AB - The carotid body (CB) is the key oxygen sensing organ. While the expression of CB specific genes is relatively well studied in animals, corresponding data for the human CB are missing. In this study we used five surgically removed human CBs to characterize the CB transcriptome with microarray and PCR analyses, and compared the results with mice data. In silico approaches demonstrated a unique gene expression profile of the human and mouse CB transcriptomes and an unexpected upregulation of both human and mouse CB genes involved in the inflammatory response compared to brain and adrenal gland data. Human CBs express most of the genes previously proposed to be involved in oxygen sensing and signalling based on animal studies, including NOX2, AMPK, CSE and oxygen sensitive K+ channels. In the TASK subfamily of K+ channels, TASK-1 is expressed in human CBs, while TASK-3 and TASK-5 are absent, although we demonstrated both TASK-1 and TASK-3 in one of the mouse reference strains. Maxi-K was expressed exclusively as the spliced variant ZERO in the human CB. In summary, the human CB transcriptome shares important features with the mouse CB, but also differs significantly in the expression of a number of CB chemosensory genes. This study provides key information for future functional investigations on the human carotid body.
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U2 - 10.1113/jphysiol.2012.231084
DO - 10.1113/jphysiol.2012.231084
M3 - Article
C2 - 22615433
AN - SCOPUS:84865651929
SN - 0022-3751
VL - 590
SP - 3807
EP - 3819
JO - Journal of Physiology
JF - Journal of Physiology
IS - 16
ER -