The H+/O ratio of proton translocation linked to the oxidation of succinate by mitochondria. Reply to a commentary

Albert L. Lehninger, Baltazar Reynafarje, Richard W. Hendler, Richard I. Shrager

Research output: Contribution to journalArticle


Costa, L.E., Reynafarje, B. and Lehninger, A.L. [(1984) J. Biol. Chem. 259, 4802-4811] have reported 'second-generation' measurements of the H+/O ratio approaching 8.0 for vectorial H+ translocation coupled to succinate oxidation by rat liver mitochondria. In a Commentary in this Journal [Krab, K., Soos, J. and Wikström, M. (1984) FEBS Lett. 178, 187-192] it was concluded that the measurements of Costa et al. significantly overestimated the true H+/O stoichiometry. It is shown here that the mathematical simulation on which Krab et al. based this claim is faulty and that data reported by Costa et al. had already excluded the criticism advanced by Krab et al. Also reported are new data, obtained under conditions in which the arguments of Krab et al. are irrelevant, which confirm that the H+/O ratio for succinate oxidation extrapolated to level flow is close to 8.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalFEBS Letters
Issue number2
Publication statusPublished - Nov 18 1985



  • Electron transport
  • Mitochondria
  • Proton
  • Stoichiometry
  • Succinate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this