The H+/O ratio of proton translocation linked to the oxidation of succinate by mitochondria. Reply to a commentary

Albert L. Lehninger, Baltazar Reynafarje, Richard W. Hendler, Richard I. Shrager

Research output: Contribution to journalArticle

Abstract

Costa, L.E., Reynafarje, B. and Lehninger, A.L. [(1984) J. Biol. Chem. 259, 4802-4811] have reported 'second-generation' measurements of the H+/O ratio approaching 8.0 for vectorial H+ translocation coupled to succinate oxidation by rat liver mitochondria. In a Commentary in this Journal [Krab, K., Soos, J. and Wikström, M. (1984) FEBS Lett. 178, 187-192] it was concluded that the measurements of Costa et al. significantly overestimated the true H+/O stoichiometry. It is shown here that the mathematical simulation on which Krab et al. based this claim is faulty and that data reported by Costa et al. had already excluded the criticism advanced by Krab et al. Also reported are new data, obtained under conditions in which the arguments of Krab et al. are irrelevant, which confirm that the H+/O ratio for succinate oxidation extrapolated to level flow is close to 8.

Original languageEnglish (US)
Pages (from-to)173-178
Number of pages6
JournalFEBS Letters
Volume192
Issue number2
DOIs
StatePublished - Nov 18 1985

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Mitochondria
Succinic Acid
Protons
Oxidation
Liver Mitochondrion
Stoichiometry
Liver
Rats

Keywords

  • Electron transport
  • Mitochondria
  • Proton
  • Stoichiometry
  • Succinate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The H+/O ratio of proton translocation linked to the oxidation of succinate by mitochondria. Reply to a commentary. / Lehninger, Albert L.; Reynafarje, Baltazar; Hendler, Richard W.; Shrager, Richard I.

In: FEBS Letters, Vol. 192, No. 2, 18.11.1985, p. 173-178.

Research output: Contribution to journalArticle

Lehninger, Albert L. ; Reynafarje, Baltazar ; Hendler, Richard W. ; Shrager, Richard I. / The H+/O ratio of proton translocation linked to the oxidation of succinate by mitochondria. Reply to a commentary. In: FEBS Letters. 1985 ; Vol. 192, No. 2. pp. 173-178.
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