The Hippo effector Yorkie activates transcription by interacting with a histone methyltransferase complex through Ncoa6

Yun Qing, Feng Yin, Wei Wang, Yonggang Zheng, Pengfei Guo, Frederick Schozer, Hua Deng, Duojia Pan

Research output: Contribution to journalArticle

Abstract

The Hippo signaling pathway regulates tissue growth in Drosophila through the transcriptional coactivator Yorkie (Yki). How Yki activates target gene transcription is poorly understood. Here, we identify Nuclear receptor coactivator 6 (Ncoa6), a subunit of the Trithoraxrelated (Trr) histone H3 lysine 4 (H3K4) methyltransferase complex, as a Yki-binding protein. Like Yki, Ncoa6 and Trr are functionally required for Hippo-mediated growth control and target gene expression. Strikingly, artificial tethering of Ncoa6 to Sd is sufficient to promote tissue growth and Yki target expression even in the absence of Yki, underscoring the importance of Yki-mediated recruitment of Ncoa6 in transcriptional activation. Consistent with the established role for the Trr complex in histone methylation, we show that Yki, Ncoa6, and Trr are required for normal H3K4 methylation at Hippo target genes. These findings shed light on Yki-mediated transcriptional regulation and uncover a potential link between chromatin modification and tissue growth.

Original languageEnglish (US)
Article numbere02564
Pages (from-to)1-13
Number of pages13
JournaleLife
Volume3
Issue numberJuly2014
DOIs
StatePublished - Jul 15 2014

Fingerprint

Nuclear Receptor Coactivators
Transcription
Methylation
Tissue
Growth
Histones
Genes
Methyltransferases
Gene expression
Transcriptional Activation
Lysine
Drosophila
Chromatin
Carrier Proteins
Chemical activation
histone methyltransferase
Gene Expression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Qing, Y., Yin, F., Wang, W., Zheng, Y., Guo, P., Schozer, F., ... Pan, D. (2014). The Hippo effector Yorkie activates transcription by interacting with a histone methyltransferase complex through Ncoa6. eLife, 3(July2014), 1-13. [e02564]. https://doi.org/10.7554/eLife.02564

The Hippo effector Yorkie activates transcription by interacting with a histone methyltransferase complex through Ncoa6. / Qing, Yun; Yin, Feng; Wang, Wei; Zheng, Yonggang; Guo, Pengfei; Schozer, Frederick; Deng, Hua; Pan, Duojia.

In: eLife, Vol. 3, No. July2014, e02564, 15.07.2014, p. 1-13.

Research output: Contribution to journalArticle

Qing, Y, Yin, F, Wang, W, Zheng, Y, Guo, P, Schozer, F, Deng, H & Pan, D 2014, 'The Hippo effector Yorkie activates transcription by interacting with a histone methyltransferase complex through Ncoa6', eLife, vol. 3, no. July2014, e02564, pp. 1-13. https://doi.org/10.7554/eLife.02564
Qing, Yun ; Yin, Feng ; Wang, Wei ; Zheng, Yonggang ; Guo, Pengfei ; Schozer, Frederick ; Deng, Hua ; Pan, Duojia. / The Hippo effector Yorkie activates transcription by interacting with a histone methyltransferase complex through Ncoa6. In: eLife. 2014 ; Vol. 3, No. July2014. pp. 1-13.
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