The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe

Adam G. Evertts, Christopher Plymire, Nancy L. Craig, Henry L. Levin

Research output: Contribution to journalArticlepeer-review

Abstract

Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.

Original languageEnglish (US)
Pages (from-to)2519-2523
Number of pages5
JournalGenetics
Volume177
Issue number4
DOIs
StatePublished - Dec 2007

ASJC Scopus subject areas

  • Genetics

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