The H-ras oncogene interferes with retinoic acid signaling and metabolism in NIH3T3 cells

K. Kosa, C. S. Jones, Luigi M De Luca

Research output: Contribution to journalArticle

Abstract

We have previously shown that retinoic acid (RA) fails to induce transglutaminase C in H-ras transformed NIH-3T3 cells. Therefore, we investigated the effect of the H-ras oncogene on the metabolism of RA and on the expression of the cellular RA-binding protein I mRNA. HPLC analysis of the media and cell extracts demonstrated that H-ras-transformed cells metabolize RA to a much lesser extent than control cells, resulting in a higher concentration of RA in H-ras cells. Although inactive in endogenous transglutaminase induction, H-ras cell-associated RA was shown to be biologically available to induce activation of a reporter construct containing a retinoid response element and in stimulating transglutaminase activity in nontransfected cells. Cellular RA-binding protein I mRNA, supposedly involved in RA storage, was significantly increased in the H-ras- transformed cells. These data demonstrate that, even though H-ras-transformed cells accumulate up to 20 fold the concentration of RA as NIH-3T3 cells, they fail to show transglutaminase induction, suggesting that H-ras interferes with signal transduction by RA.

Original languageEnglish (US)
Pages (from-to)4850-4854
Number of pages5
JournalCancer Research
Volume55
Issue number21
StatePublished - 1995
Externally publishedYes

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ras Genes
Tretinoin
Transglutaminases
NIH 3T3 Cells
Messenger RNA
Retinoids
Response Elements
Cell Extracts
Signal Transduction
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The H-ras oncogene interferes with retinoic acid signaling and metabolism in NIH3T3 cells. / Kosa, K.; Jones, C. S.; De Luca, Luigi M.

In: Cancer Research, Vol. 55, No. 21, 1995, p. 4850-4854.

Research output: Contribution to journalArticle

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