The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

Erik A. Rodriguez; Robert E. Campbell; John Y. Lin; Michael Z. Lin; Atsushi Miyawaki; Amy E. Palmer; Xiaokun Shu; Jin Zhang; Roger Y. Tsien

doi:10.1016/j.tibs.2016.09.010

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

Erik A. Rodriguez, Robert E. Campbell, John Y. Lin, Michael Z. Lin, Atsushi Miyawaki, Amy E. Palmer, Xiaokun Shu, Jin Zhang, Roger Y. Tsien

Research output: Contribution to journal › Review article › peer-review

263 Scopus citations

Abstract

Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting them for a fantastic range of applications. FPs have been modified by a combination of rational design, structure-based mutagenesis, and countless cycles of directed evolution (gene diversification followed by selection of clones with desired properties) that have collectively pushed the properties to photophysical and biochemical extremes. In this review, we provide both a summary of the progress that has been made during the past two decades, and a broad overview of the current state of FP development and applications in mammalian systems.

Original language	English (US)
Pages (from-to)	111-129
Number of pages	19
Journal	Trends in Biochemical Sciences
Volume	42
Issue number	2
DOIs	https://doi.org/10.1016/j.tibs.2016.09.010
State	Published - Feb 1 2017
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1016/j.tibs.2016.09.010

Cite this

@article{a52df8d6f8464366ac07496988822225,

title = "The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins",

abstract = "Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting them for a fantastic range of applications. FPs have been modified by a combination of rational design, structure-based mutagenesis, and countless cycles of directed evolution (gene diversification followed by selection of clones with desired properties) that have collectively pushed the properties to photophysical and biochemical extremes. In this review, we provide both a summary of the progress that has been made during the past two decades, and a broad overview of the current state of FP development and applications in mammalian systems.",

author = "Rodriguez, {Erik A.} and Campbell, {Robert E.} and Lin, {John Y.} and Lin, {Michael Z.} and Atsushi Miyawaki and Palmer, {Amy E.} and Xiaokun Shu and Jin Zhang and Tsien, {Roger Y.}",

year = "2017",

month = feb,

day = "1",

doi = "10.1016/j.tibs.2016.09.010",

language = "English (US)",

volume = "42",

pages = "111--129",

journal = "Trends in Biochemical Sciences",

issn = "0968-0004",

publisher = "Elsevier Limited",

number = "2",

}

TY - JOUR

T1 - The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

AU - Rodriguez, Erik A.

AU - Campbell, Robert E.

AU - Lin, John Y.

AU - Lin, Michael Z.

AU - Miyawaki, Atsushi

AU - Palmer, Amy E.

AU - Shu, Xiaokun

AU - Zhang, Jin

AU - Tsien, Roger Y.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting them for a fantastic range of applications. FPs have been modified by a combination of rational design, structure-based mutagenesis, and countless cycles of directed evolution (gene diversification followed by selection of clones with desired properties) that have collectively pushed the properties to photophysical and biochemical extremes. In this review, we provide both a summary of the progress that has been made during the past two decades, and a broad overview of the current state of FP development and applications in mammalian systems.

AB - Over the past 20 years, protein engineering has been extensively used to improve and modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting them for a fantastic range of applications. FPs have been modified by a combination of rational design, structure-based mutagenesis, and countless cycles of directed evolution (gene diversification followed by selection of clones with desired properties) that have collectively pushed the properties to photophysical and biochemical extremes. In this review, we provide both a summary of the progress that has been made during the past two decades, and a broad overview of the current state of FP development and applications in mammalian systems.

UR - http://www.scopus.com/inward/record.url?scp=85005915309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85005915309&partnerID=8YFLogxK

U2 - 10.1016/j.tibs.2016.09.010

DO - 10.1016/j.tibs.2016.09.010

M3 - Review article

C2 - 27814948

AN - SCOPUS:85005915309

SN - 0968-0004

VL - 42

SP - 111

EP - 129

JO - Trends in Biochemical Sciences

JF - Trends in Biochemical Sciences

IS - 2

ER -

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this