The glycosylphosphatidylinositol-anchored DFG family is essential for the insertion of galactomannan into the β-(1,3)-glucan-chitin core of the cell wall of Aspergillus fumigatus

Laetitia Muszkieta, Thierry Fontaine, Rémi Beau, Isabelle Mouyna, Marian Samuel Vogt, Jonathan Trow, Brendan P Cormack, Lars Oliver Essen, Gregory Jouvion, Jean Paul Latgé

Research output: Contribution to journalArticle

Abstract

The fungal cell wall is a complex and dynamic entity essential for the development of fungi. It is composed mainly of polysaccharides that are synthetized by protein complexes. At the cell wall level, enzyme activities are involved in postsynthesis polysaccharide modifications such as cleavage, elongation, branching, and cross-linking. Glycosylphosphatidylinositol (GPI)-anchored proteins have been shown to participate in cell wall biosynthesis and specifically in polysaccharide remodeling. Among these proteins, the DFG family plays an essential role in controlling polar growth in yeast. In the filamentous fungus and opportunistic human pathogen Aspergillus fumigatus, the DFG gene family contains seven orthologous DFG genes among which only six are expressed under in vitro growth conditions. Deletions of single DFG genes revealed that DFG3 plays the most important morphogenetic role in this gene family. A sextuple-deletion mutant resulting from the deletion of all in vitro expressed DFG genes did not contain galactomannan in the cell wall and has severe growth defects. This study has shown that DFG members are absolutely necessary for the insertion of galactomannan into the cell wall of A. fumigatus and that the proper cell wall localization of the galactomannan is essential for correct fungal morphogenesis in A. fumigatus.

Original languageEnglish (US)
Article numbere00397-19
JournalmSphere
Volume4
Issue number4
DOIs
StatePublished - Jan 1 2019

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Glycosylphosphatidylinositols
Chitin
Aspergillus fumigatus
Glucans
Cell Wall
Polysaccharides
Genes
Fungi
Growth
Proteins
Morphogenesis
galactomannan
Yeasts
Enzymes

Keywords

  • Aspergillus fumigatus
  • Cell wall
  • Glycobiology

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

The glycosylphosphatidylinositol-anchored DFG family is essential for the insertion of galactomannan into the β-(1,3)-glucan-chitin core of the cell wall of Aspergillus fumigatus. / Muszkieta, Laetitia; Fontaine, Thierry; Beau, Rémi; Mouyna, Isabelle; Vogt, Marian Samuel; Trow, Jonathan; Cormack, Brendan P; Essen, Lars Oliver; Jouvion, Gregory; Latgé, Jean Paul.

In: mSphere, Vol. 4, No. 4, e00397-19, 01.01.2019.

Research output: Contribution to journalArticle

Muszkieta, Laetitia ; Fontaine, Thierry ; Beau, Rémi ; Mouyna, Isabelle ; Vogt, Marian Samuel ; Trow, Jonathan ; Cormack, Brendan P ; Essen, Lars Oliver ; Jouvion, Gregory ; Latgé, Jean Paul. / The glycosylphosphatidylinositol-anchored DFG family is essential for the insertion of galactomannan into the β-(1,3)-glucan-chitin core of the cell wall of Aspergillus fumigatus. In: mSphere. 2019 ; Vol. 4, No. 4.
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