The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT

Chetan Bettegowda, Xin Huang, Jimmy Lin, Ian Cheong, Manu Kohli, Stephen A. Szabo, Xiaosong Zhang, Luis A. Diaz, Victor E Velculescu, Giovanni Parmigiani, Kenneth W Kinzler, Bert Vogelstein, Shibin Zhou

Research output: Contribution to journalArticle

Abstract

Bacteriolytic anti-cancer therapies employ attenuated bacterial strains that selectively proliferate within tumors. Clostridium novyi-NT spores represent one of the most promising of these agents, as they generate potent anti-tumor effects in experimental animals. We have determined the 2.55-Mb genomic sequence of C. novyi-NT, identifying a new type of transposition and 139 genes that do not have homologs in other bacteria. The genomic sequence was used to facilitate the detection of transcripts expressed at various stages of the life cycle of this bacterium in vitro as well as in infections of tumors in vivo. Through this analysis, we found that C. novyi-NT spores contained mRNA and that the spore transcripts were distinct from those in vegetative forms of the bacterium.

Original languageEnglish (US)
Pages (from-to)1573-1580
Number of pages8
JournalNature Biotechnology
Volume24
Issue number12
DOIs
StatePublished - Dec 2006

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Clostridium
Transcriptome
Tumors
Bacteria
Genes
Spores
Genome
Neoplasms
Life cycle
Animals
Life Cycle Stages
Messenger RNA
Infection
Therapeutics

ASJC Scopus subject areas

  • Microbiology

Cite this

The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT. / Bettegowda, Chetan; Huang, Xin; Lin, Jimmy; Cheong, Ian; Kohli, Manu; Szabo, Stephen A.; Zhang, Xiaosong; Diaz, Luis A.; Velculescu, Victor E; Parmigiani, Giovanni; Kinzler, Kenneth W; Vogelstein, Bert; Zhou, Shibin.

In: Nature Biotechnology, Vol. 24, No. 12, 12.2006, p. 1573-1580.

Research output: Contribution to journalArticle

Bettegowda, C, Huang, X, Lin, J, Cheong, I, Kohli, M, Szabo, SA, Zhang, X, Diaz, LA, Velculescu, VE, Parmigiani, G, Kinzler, KW, Vogelstein, B & Zhou, S 2006, 'The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT', Nature Biotechnology, vol. 24, no. 12, pp. 1573-1580. https://doi.org/10.1038/nbt1256
Bettegowda, Chetan ; Huang, Xin ; Lin, Jimmy ; Cheong, Ian ; Kohli, Manu ; Szabo, Stephen A. ; Zhang, Xiaosong ; Diaz, Luis A. ; Velculescu, Victor E ; Parmigiani, Giovanni ; Kinzler, Kenneth W ; Vogelstein, Bert ; Zhou, Shibin. / The genome and transcriptomes of the anti-tumor agent Clostridium novyi-NT. In: Nature Biotechnology. 2006 ; Vol. 24, No. 12. pp. 1573-1580.
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