The genetic program of hematopoietic stem cells

Robert L. Phillips; Robin E. Ernst; Brian Brunk; Natalia Ivanova; Mark A. Mahan; Julia K. Deanehan; Kateri A. Moore; G. Christian Overton; Ihor R. Lemischka

doi:10.1126/science.288.5471.1635

The genetic program of hematopoietic stem cells

Robert L. Phillips, Robin E. Ernst, Brian Brunk, Natalia Ivanova, Mark A. Mahan, Julia K. Deanehan, Kateri A. Moore, G. Christian Overton, Ihor R. Lemischka

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354 Scopus citations

Abstract

Blood cell production originates from a rare population of multipotent, self-renewing stem cells. A genome-wide gene expression analysis was performed in order to define regulatory pathways in stem cells as well as their global genetic program. Subtracted complementary DNA libraries from highly purified murine fetal liver stem cells were analyzed with bioinformatic and array hybridization strategies. A large percentage of the several thousand gene products that have been characterized correspond to previously undescribed molecules with properties suggestive of regulatory functions. The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell.

Original language	English (US)
Pages (from-to)	1635-1640
Number of pages	6
Journal	Science
Volume	288
Issue number	5471
DOIs	https://doi.org/10.1126/science.288.5471.1635
State	Published - Jun 2 2000
Externally published	Yes

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title = "The genetic program of hematopoietic stem cells",

abstract = "Blood cell production originates from a rare population of multipotent, self-renewing stem cells. A genome-wide gene expression analysis was performed in order to define regulatory pathways in stem cells as well as their global genetic program. Subtracted complementary DNA libraries from highly purified murine fetal liver stem cells were analyzed with bioinformatic and array hybridization strategies. A large percentage of the several thousand gene products that have been characterized correspond to previously undescribed molecules with properties suggestive of regulatory functions. The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell.",

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doi = "10.1126/science.288.5471.1635",

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AU - Ivanova, Natalia

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AU - Deanehan, Julia K.

AU - Moore, Kateri A.

AU - Overton, G. Christian

AU - Lemischka, Ihor R.

PY - 2000/6/2

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AB - Blood cell production originates from a rare population of multipotent, self-renewing stem cells. A genome-wide gene expression analysis was performed in order to define regulatory pathways in stem cells as well as their global genetic program. Subtracted complementary DNA libraries from highly purified murine fetal liver stem cells were analyzed with bioinformatic and array hybridization strategies. A large percentage of the several thousand gene products that have been characterized correspond to previously undescribed molecules with properties suggestive of regulatory functions. The complete data, available in a biological process-oriented database, represent the molecular phenotype of the hematopoietic stem cell.

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The genetic program of hematopoietic stem cells

Abstract

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