TY - JOUR
T1 - The Generation of Chemiluminescence (CL) by Phagocytic Cells
AU - Trush, Michael A.
AU - Wilson, Mark E.
AU - van Dyke, Knox
N1 - Funding Information:
Work done by the authors has been supported, in part, by grants from the following sources: West Virginia University Senate Grant; West Virginia University Institutional Grant; West Virginia Heart Association; National Institute of Health Training Grant T 32 GM07039.
PY - 1978/1/1
Y1 - 1978/1/1
N2 - This chapter presents the methods involved in generating chemiluminescence (CL) from phagocytic cells and the procedures involved in utilizing CL as both an index to study defects of phagocytosis of both cellular and humoral origin, and to study the interaction of pharmacologic and toxicologic agents with phagocytic cells. The generation of CL is characteristic of phagocytic cells other than neutrophiles, including alveolar and peritoneal macrophages, monocytes, and eosinophils. The use of CL as an index to study the pharmacologic and/ortoxicologic effects of agents on phagocytic cells has widespread application and potential. The measurement of CL by phagocytic cells is found to be a reliable and reproducible parameter by which phagocytosis-associated events can be assessed and this system has widespread applicability to a variety of problems, both scientific and clinical in nature.
AB - This chapter presents the methods involved in generating chemiluminescence (CL) from phagocytic cells and the procedures involved in utilizing CL as both an index to study defects of phagocytosis of both cellular and humoral origin, and to study the interaction of pharmacologic and toxicologic agents with phagocytic cells. The generation of CL is characteristic of phagocytic cells other than neutrophiles, including alveolar and peritoneal macrophages, monocytes, and eosinophils. The use of CL as an index to study the pharmacologic and/ortoxicologic effects of agents on phagocytic cells has widespread application and potential. The measurement of CL by phagocytic cells is found to be a reliable and reproducible parameter by which phagocytosis-associated events can be assessed and this system has widespread applicability to a variety of problems, both scientific and clinical in nature.
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U2 - 10.1016/0076-6879(78)57041-9
DO - 10.1016/0076-6879(78)57041-9
M3 - Article
AN - SCOPUS:77956987316
VL - 57
SP - 462
EP - 494
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -