The Gene for a Variant Form of the Polyadenylation Protein CstF-64 Is on Chromosome 19 and Is Expressed in Pachytene Spermatocytes in Mice

Brinda Dass, K. Wyatt McMahon, Nancy A. Jenkins, Debra J. Gilbert, Neal G. Copeland, Clinton C. MacDonald

Research output: Contribution to journalArticle

Abstract

Many mRNAs in male germ cells lack the canonical AAUAAA but are normally polyadenylated (Wallace, A. M., Dass, B., Ravnik, S. E., Tonk, V., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., and MacDonald, C. C. (1999) Proc. Natl. Acad Sci. U. S. A. 96, 6763-6768). Previously, we demonstrated the presence of two distinct forms of the Mr 64,000 protein of the cleavage stimulation factor (CstF-64) in mouse male germ cells and in brain, a somatic Mr 64,000 form and a variant Mr 70,000 form. The variant form was specific to meiotic and post-meiotic germ cells. We localized the gene for the somatic CstF-64 to the X chromosome, which would be inactivated during male meiosis. This suggested that the variant CstF-64 was an autosomal homolog activated during that time. We have named the variant form "τ CstF-64," and we describe here the cloning and characterization of the mouse τCstF-64 cDNA, which maps to chromosome 19. The mouse τCstF-64 protein fits the criteria of the variant CstF-64, including antibody reactivity, size, germ cell expression, and a common proteolytic digest pattern with τCstF-64 from testis. Features of mτCstF-64 that might allow it to promote the germ cell pattern of polyadenylation include a Pro → Ser substitution in the RNA-binding domain and significant changes in the region that interacts with CstF-77.

Original languageEnglish (US)
Pages (from-to)8044-8050
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number11
DOIs
StatePublished - Mar 16 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The Gene for a Variant Form of the Polyadenylation Protein CstF-64 Is on Chromosome 19 and Is Expressed in Pachytene Spermatocytes in Mice'. Together they form a unique fingerprint.

  • Cite this