The Gene Encoding Murine α1,3-Galactosyltransferase Is Expressed in Female Germ Cells but Not in Male Germ Cells

Daniel S. Johnston, Joel H. Shaper, Nancy L. Shaper, David H. Joziasse, William W Wright

Research output: Contribution to journalArticle

Abstract

An essential step in murine fertilization is the binding of acrosome-intact sperm to specific O-linked glycans on zona pellucida glycoprotein 3 (ZP3). While there is agreement on the primary role of O-linked glycans in sperm-ZP3 binding, there is a striking lack of consensus on both the terminal monosaccharide(s) required for a functional binding site and the cognate protein on the sperm cell surface that recognizes this glycan. Much current debate centers on the essential role of nonreducing terminal N -acetyl-glucosaminyl or alternatively, α-galactosyl residues, to form a functional sperm binding ligand. Relevant to this debate, we demonstrated that α1,3-galactosyltransferase (α3-GT), which adds nonreducing terminal α-galactosyl residues to glycans, is not expressed in murine spermatocytes or spermatids. The objectives of this study were to determine whether α3-GT is expressed in female germ cells and to compare the pattern of expression of two other terminal glycosyltransferases, β1,4-galactosyltransferase (β4-GT) and α2,6-sialyltransferase (α6-ST), between male and female germ cells. Total RNA was isolated from growing oocytes obtained from 15-day-old animals, fully grown oocytes, and eggs as wall as spermatogonia, spermatocytes, and spermatids. The presence of α3-GT, β4-GT, and α6-ST mRNAs was analyzed by an RT-PCR-based assay. Our data demonstrate that the α3-GT gene is expressed in female germ cells, but not in male germ cells. In contrast, both β4-GT and α6-ST are expressed during oogenesis and spermatogenesis. This differential expression of α3-GT in female germ cells is consistent with the model of sperm-egg binding in which a nonreducing terminal α-galactosyl residue is required for a functional determinant on ZP3 and with our hypothesis that the biological significance for the suppression of α3-GT expression in male germ cells is to prevent sperm-sperm aggregation.

Original languageEnglish (US)
Pages (from-to)224-232
Number of pages9
JournalDevelopmental Biology
Volume171
Issue number1
DOIs
StatePublished - Sep 1995

Fingerprint

Galactosyltransferases
Germ Cells
Spermatozoa
Sialyltransferases
Genes
Polysaccharides
Spermatocytes
Spermatids
Oocytes
Acrosome
Oogenesis
Glycosyltransferases
Spermatogonia
Monosaccharides
Spermatogenesis
Fertilization
Eggs
Ovum
Binding Sites
RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

The Gene Encoding Murine α1,3-Galactosyltransferase Is Expressed in Female Germ Cells but Not in Male Germ Cells. / Johnston, Daniel S.; Shaper, Joel H.; Shaper, Nancy L.; Joziasse, David H.; Wright, William W.

In: Developmental Biology, Vol. 171, No. 1, 09.1995, p. 224-232.

Research output: Contribution to journalArticle

Johnston, Daniel S. ; Shaper, Joel H. ; Shaper, Nancy L. ; Joziasse, David H. ; Wright, William W. / The Gene Encoding Murine α1,3-Galactosyltransferase Is Expressed in Female Germ Cells but Not in Male Germ Cells. In: Developmental Biology. 1995 ; Vol. 171, No. 1. pp. 224-232.
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