The GATA transcription factor GtaC regulates early developmental gene expression dynamics in Dictyostelium

Balaji Santhanam; Huaqing Cai; Peter N. Devreotes; Gad Shaulsky; Mariko Katoh-Kurasawa

doi:10.1038/ncomms8551

The GATA transcription factor GtaC regulates early developmental gene expression dynamics in Dictyostelium

Balaji Santhanam, Huaqing Cai, Peter N. Devreotes, Gad Shaulsky, Mariko Katoh-Kurasawa

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

In many systems, including the social amoeba Dictyostelium discoideum, development is often marked by dynamic morphological and transcriptional changes orchestrated by key transcription factors. However, efforts to examine sequential genome-wide changes of gene regulation in developmental processes have been fairly limited. Here we report the developmental regulatory dynamics of GtaC, a GATA-type zinc-finger transcription factor, through the analyses of serial ChIP-and RNA-sequencing data. GtaC is essential for developmental progression, decoding extracellular cAMP pulses during early development and may play a role in mediating cell-type differentiation at later stages. We find that GtaC exhibits temporally distinctive DNA-binding patterns concordant with each developmental stage. We identify direct GtaC targets and observe cotemporaneous GtaC-binding and developmental expression regulation. Our results suggest that GtaC regulates multiple physiological processes as Dictyostelium transitions from a group of unicellular amoebae to an integrated multicellular organism.

Original language	English (US)
Article number	7551
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms8551
State	Published - Jul 6 2015

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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title = "The GATA transcription factor GtaC regulates early developmental gene expression dynamics in Dictyostelium",

abstract = "In many systems, including the social amoeba Dictyostelium discoideum, development is often marked by dynamic morphological and transcriptional changes orchestrated by key transcription factors. However, efforts to examine sequential genome-wide changes of gene regulation in developmental processes have been fairly limited. Here we report the developmental regulatory dynamics of GtaC, a GATA-type zinc-finger transcription factor, through the analyses of serial ChIP-and RNA-sequencing data. GtaC is essential for developmental progression, decoding extracellular cAMP pulses during early development and may play a role in mediating cell-type differentiation at later stages. We find that GtaC exhibits temporally distinctive DNA-binding patterns concordant with each developmental stage. We identify direct GtaC targets and observe cotemporaneous GtaC-binding and developmental expression regulation. Our results suggest that GtaC regulates multiple physiological processes as Dictyostelium transitions from a group of unicellular amoebae to an integrated multicellular organism.",

author = "Balaji Santhanam and Huaqing Cai and Devreotes, {Peter N.} and Gad Shaulsky and Mariko Katoh-Kurasawa",

note = "Funding Information: We thank Gregor Rot, Marko Toplak and Blazˇ Zupan from the University of Ljubljana for assistance with data management and analyses. We also thank Zhangdong Liu at the Department of Pediatrics, Baylor College of Medicine and Marinka Zˇitnik and Blazˇ Zupan at the University of Ljubljana for useful suggestions and helpful discussions. This work was supported by the National institutes of Health {\textquoteleft}the Dictyostelium Functional Genomics Program Project Grant{\textquoteright} (P01 HD39691) to G.S., R01 GM28007 and R01 GM34933 to P.N.D. and Helen Hay Whitney postdoctoral fellowship to H.C. Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited.",

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AU - Santhanam, Balaji

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AU - Devreotes, Peter N.

AU - Shaulsky, Gad

AU - Katoh-Kurasawa, Mariko

N1 - Funding Information: We thank Gregor Rot, Marko Toplak and Blazˇ Zupan from the University of Ljubljana for assistance with data management and analyses. We also thank Zhangdong Liu at the Department of Pediatrics, Baylor College of Medicine and Marinka Zˇitnik and Blazˇ Zupan at the University of Ljubljana for useful suggestions and helpful discussions. This work was supported by the National institutes of Health ‘the Dictyostelium Functional Genomics Program Project Grant’ (P01 HD39691) to G.S., R01 GM28007 and R01 GM34933 to P.N.D. and Helen Hay Whitney postdoctoral fellowship to H.C. Publisher Copyright: © 2015 Macmillan Publishers Limited.

PY - 2015/7/6

Y1 - 2015/7/6

N2 - In many systems, including the social amoeba Dictyostelium discoideum, development is often marked by dynamic morphological and transcriptional changes orchestrated by key transcription factors. However, efforts to examine sequential genome-wide changes of gene regulation in developmental processes have been fairly limited. Here we report the developmental regulatory dynamics of GtaC, a GATA-type zinc-finger transcription factor, through the analyses of serial ChIP-and RNA-sequencing data. GtaC is essential for developmental progression, decoding extracellular cAMP pulses during early development and may play a role in mediating cell-type differentiation at later stages. We find that GtaC exhibits temporally distinctive DNA-binding patterns concordant with each developmental stage. We identify direct GtaC targets and observe cotemporaneous GtaC-binding and developmental expression regulation. Our results suggest that GtaC regulates multiple physiological processes as Dictyostelium transitions from a group of unicellular amoebae to an integrated multicellular organism.

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The GATA transcription factor GtaC regulates early developmental gene expression dynamics in Dictyostelium

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