The function of guanylate cyclase 1 and guanylate cyclase 2 in rod and cone photoreceptors

Wolfgang Baehr, Sukanya Karan, Tadao Maeda, Dong Gen Luo, Sha Li, J. Darin Bronson, Carl B. Watt, King-Wai Yau, Jeanne M. Frederick, Krzysztof Palczewski

Research output: Contribution to journalArticle

Abstract

Retinal guanylate cyclases 1 and 2 (GC1 and GC2) are responsible for synthesis of cyclic GMP in rods and cones, but their individual contributions to phototransduction are unknown. We report here that the deletion of both GC1 and GC2 rendered rod and cone photoreceptors nonfunctional and unstable. In the rod outer segments of GC double knock-out mice, guanylate cyclase-activating proteins 1 and 2, and cyclic GMP phosphodiesterase were undetectable, although rhodopsin and transducin α-subunit were mostly unaffected. Outer segment membranes of GC1-/- and GC double knock-out cones were destabilized and devoid of cone transducin (α- and γ-subunits), cone phosphodiesterase, and G protein-coupled receptor kinase 1, whereas cone pigments were present at reduced levels. Real time reverse transcription-PCR analyses demonstrated normal RNA transcript levels for the down regulated proteins, indicating that down-regulation is posttranslational. We interpret these results to demonstrate an intrinsic requirement of GCs for stability and/or transport of a set of membrane-associated phototransduction proteins.

Original languageEnglish (US)
Pages (from-to)8837-8847
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number12
DOIs
StatePublished - Mar 23 2007

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Guanylate Cyclase-Activating Proteins
Transducin
Light Signal Transduction
Retinal Cone Photoreceptor Cells
Retinal Rod Photoreceptor Cells
Vertebrate Photoreceptor Cells
Guanylate Cyclase
Cyclic GMP
Phosphoric Diester Hydrolases
Cones
G-Protein-Coupled Receptor Kinase 1
Rod Cell Outer Segment
Rhodopsin
Knockout Mice
Reverse Transcription
Membrane Proteins
Down-Regulation
RNA
Polymerase Chain Reaction
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Baehr, W., Karan, S., Maeda, T., Luo, D. G., Li, S., Bronson, J. D., ... Palczewski, K. (2007). The function of guanylate cyclase 1 and guanylate cyclase 2 in rod and cone photoreceptors. Journal of Biological Chemistry, 282(12), 8837-8847. https://doi.org/10.1074/jbc.M610369200

The function of guanylate cyclase 1 and guanylate cyclase 2 in rod and cone photoreceptors. / Baehr, Wolfgang; Karan, Sukanya; Maeda, Tadao; Luo, Dong Gen; Li, Sha; Bronson, J. Darin; Watt, Carl B.; Yau, King-Wai; Frederick, Jeanne M.; Palczewski, Krzysztof.

In: Journal of Biological Chemistry, Vol. 282, No. 12, 23.03.2007, p. 8837-8847.

Research output: Contribution to journalArticle

Baehr, W, Karan, S, Maeda, T, Luo, DG, Li, S, Bronson, JD, Watt, CB, Yau, K-W, Frederick, JM & Palczewski, K 2007, 'The function of guanylate cyclase 1 and guanylate cyclase 2 in rod and cone photoreceptors', Journal of Biological Chemistry, vol. 282, no. 12, pp. 8837-8847. https://doi.org/10.1074/jbc.M610369200
Baehr, Wolfgang ; Karan, Sukanya ; Maeda, Tadao ; Luo, Dong Gen ; Li, Sha ; Bronson, J. Darin ; Watt, Carl B. ; Yau, King-Wai ; Frederick, Jeanne M. ; Palczewski, Krzysztof. / The function of guanylate cyclase 1 and guanylate cyclase 2 in rod and cone photoreceptors. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 12. pp. 8837-8847.
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