The frequency of HIV-I drug resistance mutations among treatment-naïve individuals at a tertiary care centre in south India

A. J. Kandathil; R. Kannangai; O. C. Abraham; P. Rupali; S. A. Pulimood; V. P. Verghese; P. Grant; D. Pillay; G. Sridharan

doi:10.1258/ijsa.2008.008403

The frequency of HIV-I drug resistance mutations among treatment-naïve individuals at a tertiary care centre in south India

A. J. Kandathil, R. Kannangai, O. C. Abraham, P. Rupali, S. A. Pulimood, V. P. Verghese, P. Grant, D. Pillay, G. Sridharan

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Antiretroviral treatment (ART) use in India requires information on baseline drug resistance mutations and polymorphisms in the protease (Pr) and reverse transcriptase (RT) genes of HIV-1 strains from treatment-naïve individuals. We report resistance predictor mutations and polymorphisms in the Pr and the RT sequence of non-clade B HIV-1 strains from ART naïve individuals. The genotypic resistance assay was done on 93 treatment-naïve individuals. The sequences were analysed by Stanford HIV drug resistance data for genotypic drug resistance analysis and REGA HIV-1 subtyping tool. Phylogenetic tree was generated with MEGA 4 for quality control. Ninety-two strains belonged to clade C and one to clade A (A1). Amino acid substitutions were seen at positions associated with drug resistance in Pr gene - 10, 24, 74 (each 3%) and position 82 (11%). Substitutions were seen at positions 41 (1%), 100 (1%), 101 (6%), 103 (2%), 179 (6%) and 181 (1%) of the RT sequence known to confer drug resistance in clade B. Polymorphisms in HIV-1 pol gene among treatment-naïve individuals were similar when compared with previous data. One strain each had Y181C substitution, T74S and E35G substitutions in the Pr and one had A98G, K101R and L210FL substitutions in RT.

Original language	English (US)
Pages (from-to)	522-526
Number of pages	5
Journal	International Journal of STD and AIDS
Volume	20
Issue number	8
DOIs	https://doi.org/10.1258/ijsa.2008.008403
State	Published - Aug 1 2008
Externally published	Yes

Keywords

Drug resistance
Genotyping testing
HIV-1
India
Non-clade B

ASJC Scopus subject areas

Dermatology
Public Health, Environmental and Occupational Health
Pharmacology (medical)
Infectious Diseases

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10.1258/ijsa.2008.008403

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title = "The frequency of HIV-I drug resistance mutations among treatment-na{\"i}ve individuals at a tertiary care centre in south India",

abstract = "Antiretroviral treatment (ART) use in India requires information on baseline drug resistance mutations and polymorphisms in the protease (Pr) and reverse transcriptase (RT) genes of HIV-1 strains from treatment-na{\"i}ve individuals. We report resistance predictor mutations and polymorphisms in the Pr and the RT sequence of non-clade B HIV-1 strains from ART na{\"i}ve individuals. The genotypic resistance assay was done on 93 treatment-na{\"i}ve individuals. The sequences were analysed by Stanford HIV drug resistance data for genotypic drug resistance analysis and REGA HIV-1 subtyping tool. Phylogenetic tree was generated with MEGA 4 for quality control. Ninety-two strains belonged to clade C and one to clade A (A1). Amino acid substitutions were seen at positions associated with drug resistance in Pr gene - 10, 24, 74 (each 3%) and position 82 (11%). Substitutions were seen at positions 41 (1%), 100 (1%), 101 (6%), 103 (2%), 179 (6%) and 181 (1%) of the RT sequence known to confer drug resistance in clade B. Polymorphisms in HIV-1 pol gene among treatment-na{\"i}ve individuals were similar when compared with previous data. One strain each had Y181C substitution, T74S and E35G substitutions in the Pr and one had A98G, K101R and L210FL substitutions in RT.",

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AU - Kandathil, A. J.

AU - Kannangai, R.

AU - Abraham, O. C.

AU - Rupali, P.

AU - Pulimood, S. A.

AU - Verghese, V. P.

AU - Grant, P.

AU - Pillay, D.

AU - Sridharan, G.

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The frequency of HIV-I drug resistance mutations among treatment-naïve individuals at a tertiary care centre in south India

Abstract

Keywords

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