The formation of granules in the bronchiolar Clara cells of the rat. 1. Electron microscopy

Charles Kuhn, Lawrence A. Callaway, Frederic B Askin

Research output: Contribution to journalArticle

Abstract

A morphologic study of bronchiolar Clara cells used rat lungs fixed by vascular perfusion or by inflation through the airways. Clara cells contained membrane-limited granules which had a crystalline structure with an 80 Å periodicity when fixed by perfusion. The crystalline structure was infrequently seen after inflation fixation. The smooth endoplasmic reticulum was highly developed in the apex of Clara cells and appeared to be a major site of formation of the characteristic granules. Some nascent granules were also seen in association with the Golgi apparatus. Perfusion fixation retained a bronchiolar lining layer which was denser than the alveolar lining layer in the same sections and which resembled the matrix of Clara cells in opacity. One explanation for the difference between the bronchiolar and alveolar linings may be that the bronchiolar lining is derived in part from the secretions of Clara cells. Images suggesting exocytosis, albeit infrequent, support this suggestion.

Original languageEnglish (US)
Pages (from-to)387-400
Number of pages14
JournalJournal of Ultrasructure Research
Volume49
Issue number3
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

Electron Microscopy
Perfusion
Economic Inflation
Smooth Endoplasmic Reticulum
Exocytosis
Golgi Apparatus
Periodicity
Blood Vessels
Cell Membrane
Lung

ASJC Scopus subject areas

  • Molecular Biology
  • Anatomy

Cite this

The formation of granules in the bronchiolar Clara cells of the rat. 1. Electron microscopy. / Kuhn, Charles; Callaway, Lawrence A.; Askin, Frederic B.

In: Journal of Ultrasructure Research, Vol. 49, No. 3, 1974, p. 387-400.

Research output: Contribution to journalArticle

@article{99a785fc8c124ab8ab23030bc99ccdae,
title = "The formation of granules in the bronchiolar Clara cells of the rat. 1. Electron microscopy",
abstract = "A morphologic study of bronchiolar Clara cells used rat lungs fixed by vascular perfusion or by inflation through the airways. Clara cells contained membrane-limited granules which had a crystalline structure with an 80 {\AA} periodicity when fixed by perfusion. The crystalline structure was infrequently seen after inflation fixation. The smooth endoplasmic reticulum was highly developed in the apex of Clara cells and appeared to be a major site of formation of the characteristic granules. Some nascent granules were also seen in association with the Golgi apparatus. Perfusion fixation retained a bronchiolar lining layer which was denser than the alveolar lining layer in the same sections and which resembled the matrix of Clara cells in opacity. One explanation for the difference between the bronchiolar and alveolar linings may be that the bronchiolar lining is derived in part from the secretions of Clara cells. Images suggesting exocytosis, albeit infrequent, support this suggestion.",
author = "Charles Kuhn and Callaway, {Lawrence A.} and Askin, {Frederic B}",
year = "1974",
doi = "10.1016/S0022-5320(74)90052-5",
language = "English (US)",
volume = "49",
pages = "387--400",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - The formation of granules in the bronchiolar Clara cells of the rat. 1. Electron microscopy

AU - Kuhn, Charles

AU - Callaway, Lawrence A.

AU - Askin, Frederic B

PY - 1974

Y1 - 1974

N2 - A morphologic study of bronchiolar Clara cells used rat lungs fixed by vascular perfusion or by inflation through the airways. Clara cells contained membrane-limited granules which had a crystalline structure with an 80 Å periodicity when fixed by perfusion. The crystalline structure was infrequently seen after inflation fixation. The smooth endoplasmic reticulum was highly developed in the apex of Clara cells and appeared to be a major site of formation of the characteristic granules. Some nascent granules were also seen in association with the Golgi apparatus. Perfusion fixation retained a bronchiolar lining layer which was denser than the alveolar lining layer in the same sections and which resembled the matrix of Clara cells in opacity. One explanation for the difference between the bronchiolar and alveolar linings may be that the bronchiolar lining is derived in part from the secretions of Clara cells. Images suggesting exocytosis, albeit infrequent, support this suggestion.

AB - A morphologic study of bronchiolar Clara cells used rat lungs fixed by vascular perfusion or by inflation through the airways. Clara cells contained membrane-limited granules which had a crystalline structure with an 80 Å periodicity when fixed by perfusion. The crystalline structure was infrequently seen after inflation fixation. The smooth endoplasmic reticulum was highly developed in the apex of Clara cells and appeared to be a major site of formation of the characteristic granules. Some nascent granules were also seen in association with the Golgi apparatus. Perfusion fixation retained a bronchiolar lining layer which was denser than the alveolar lining layer in the same sections and which resembled the matrix of Clara cells in opacity. One explanation for the difference between the bronchiolar and alveolar linings may be that the bronchiolar lining is derived in part from the secretions of Clara cells. Images suggesting exocytosis, albeit infrequent, support this suggestion.

UR - http://www.scopus.com/inward/record.url?scp=0016353226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016353226&partnerID=8YFLogxK

U2 - 10.1016/S0022-5320(74)90052-5

DO - 10.1016/S0022-5320(74)90052-5

M3 - Article

C2 - 4616101

AN - SCOPUS:0016353226

VL - 49

SP - 387

EP - 400

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

IS - 3

ER -