The filamentous phage (Ff) as vectors for recombinant DNA- a review

Norton D. Zinder; Jef D. Boeke

doi:10.1016/0378-1119(82)90183-4

The filamentous phage (Ff) as vectors for recombinant DNA- a review

Norton D. Zinder, Jef D. Boeke

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

Derivatives of filamentous phage, f1, fd, and M 13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.

Original language	English (US)
Pages (from-to)	1-10
Number of pages	10
Journal	Gene
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(82)90183-4
State	Published - Jan 1 1982
Externally published	Yes

Keywords

DNA preparation method
Single-stranded cloning vehicles
deletion

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0378-1119(82)90183-4

Cite this

@article{1794c33a521346d7bdc8551928773c90,

title = "The filamentous phage (Ff) as vectors for recombinant DNA- a review",

abstract = "Derivatives of filamentous phage, f1, fd, and M 13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the {"}cook-book{"} form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.",

keywords = "DNA preparation method, Single-stranded cloning vehicles, deletion",

author = "Zinder, {Norton D.} and Boeke, {Jef D.}",

year = "1982",

month = jan,

day = "1",

doi = "10.1016/0378-1119(82)90183-4",

language = "English (US)",

volume = "19",

pages = "1--10",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - The filamentous phage (Ff) as vectors for recombinant DNA- a review

AU - Zinder, Norton D.

AU - Boeke, Jef D.

PY - 1982/1/1

Y1 - 1982/1/1

N2 - Derivatives of filamentous phage, f1, fd, and M 13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.

AB - Derivatives of filamentous phage, f1, fd, and M 13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.

KW - DNA preparation method

KW - Single-stranded cloning vehicles

KW - deletion

UR - http://www.scopus.com/inward/record.url?scp=0020457545&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020457545&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(82)90183-4

DO - 10.1016/0378-1119(82)90183-4

M3 - Article

C2 - 6292041

AN - SCOPUS:0020457545

SN - 0378-1119

VL - 19

SP - 1

EP - 10

JO - Gene

JF - Gene

IS - 1

ER -

The filamentous phage (Ff) as vectors for recombinant DNA- a review

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this