The filamentous phage (Ff) as vectors for recombinant DNA- a review

Norton D. Zinder, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

Derivatives of filamentous phage, f1, fd, and M 13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalGene
Volume19
Issue number1
DOIs
StatePublished - Jan 1 1982

Keywords

  • DNA preparation method
  • Single-stranded cloning vehicles
  • deletion

ASJC Scopus subject areas

  • Genetics

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