The expression of virus-specific nucleotide sequences in rous sarcoma cells

E. A. Ratovitski, J. D. Shaposhnikov, F. Bobrov Yu., O. K. Kuznetsov

Research output: Contribution to journalArticle

Abstract

Poly(A)-enriched virus-specific mRNAs (v-mRNA) are revealed in the subcellular fractions (nuclei, mitochondria, polyribosomes and cytosol) from chicken and hamster Rous sarcoma cells. The qualitative and quantitative differences in v-mRNA populations from permissive and non-permissive tumors are shown. The sedimentation analysis of v-mRNAs allowed to ascertain the presence of three molecular classes of v-mRNA in nuclei and cytoplasm of chicken Rous sarcoma cells (35S, 28S and 22S, and 35S, 26-28S and 20S, respectively). While two size classes (33S and 20S) are shown in hamster Rous sarcoma nuclei, only 24S v-mRNA is revealed in the cytoplasm of its tumor tissue. Thus, the oncovirus gene expression is restricted in the non-permissive tumor cells, unlike of the permissive chicken Rous sarcoma cells, both in the level of transcription, and in the processing and transfer v-mRNAs from nucleus to cytoplasm. The virus gene function peculiarities in the tumors of birds and mammals are discussed.

Original languageEnglish (US)
Pages (from-to)413-422
Number of pages10
JournalNeoplasma
Volume28
Issue number4
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research

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  • Cite this

    Ratovitski, E. A., Shaposhnikov, J. D., Bobrov Yu., F., & Kuznetsov, O. K. (1981). The expression of virus-specific nucleotide sequences in rous sarcoma cells. Neoplasma, 28(4), 413-422.