The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos

Shuji Ueda, Becky Tu-Sekine, Minoru Yamanoue, Daniel M. Raben, Yasuhito Shirai

Research output: Contribution to journalArticle


Background: Diacylglycerol kinase (DGK) is a key enzyme that regulates diacylglycerol (DG) turnover and is involved in a variety of physiological functions. The isoform DGKθ has a unique domain structure and is the sole member of type V DGK. To reveal the spatial and temporal expression of DGKθ we performed immunohistochemical staining on paraffin sections of mouse embryos. Results: At an early stage of development (E10.5 and 11.5), the expression of DGKθ was prominently detected in the brain, spinal cord, dorsal root ganglion, and limb bud, and was also moderately detected in the bulbus cordis and the primordium of the liver and gut. At later stages (E12.5 and 14.5), DGKθ expression persisted or increased in the neocortex, epithalamus, hypothalamus, medulla oblongata, and pons. DGKθ was also evident in the epidermis, and nearly all epithelia of the oropharyngeal membrane, digestive tract, and bronchea. At prenatal developmental stages (E16.5 and E18.5), the expression pattern of DGKθ was maintained in the central nervous system, intestine, and kidney, but was attenuated in the differentiated epidermis. Conclusion: These results suggest that DGKθ may play important physiological roles not only in the brain, but also in diverse organs and tissues during the embryonic stages.

Original languageEnglish (US)
Article number35
JournalBMC Developmental Biology
Issue number1
StatePublished - Oct 4 2013


  • Central nerve system
  • Diacylglycerol kinase
  • Embryonic development
  • Epidermis

ASJC Scopus subject areas

  • Developmental Biology

Fingerprint Dive into the research topics of 'The expression of diacylglycerol kinase theta during the organogenesis of mouse embryos'. Together they form a unique fingerprint.

  • Cite this