The entomopathogenic fungus Beauveria bassiana activate toll and JAK-STAT pathway-controlled effector genes and anti-dengue activity in Aedes aegypti

Yuemei Dong, James C. Morton, Jose Luis Ramirez, Jayme A. Souza-Neto, George Dimopoulos

Research output: Contribution to journalArticle

Abstract

Here we investigated the effect of Beauveria bassiana infection on the survival of Aedes aegypti mosquitoes and the modulation of their susceptibility to dengue virus infection. Application of B.bassiana caused a reduction in the life span of A.aegypti and hindered dengue virus replication in the mosquito midgut. Fungus infection induced the expression a variety anti-microbial and dengue virus restriction factor genes. Transient reverse genetic analyses showed that the JAK-STAT pathway is implicated anti-fungal defense of Aedes mosquitoes. Our data suggest that this B.bassiana-mediated anti-dengue activity is likely to be at least partly indirectly mediated through the activation of the mosquito's anti-dengue Toll and JAK-STAT pathways.

Original languageEnglish (US)
Pages (from-to)126-132
Number of pages7
JournalInsect Biochemistry and Molecular Biology
Volume42
Issue number2
DOIs
StatePublished - Feb 2012

Fingerprint

Beauveria
dengue
Dengue
Aedes
Beauveria bassiana
entomopathogenic fungi
Aedes aegypti
Culicidae
Fungi
Viruses
Dengue virus
Dengue Virus
Genes
genes
infection
Reverse Genetics
Mycoses
Virus Diseases
Virus Replication
virus replication

Keywords

  • Aedes aegypti
  • Beauveria bassiana
  • Dengue virus
  • Innate immunity

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

The entomopathogenic fungus Beauveria bassiana activate toll and JAK-STAT pathway-controlled effector genes and anti-dengue activity in Aedes aegypti. / Dong, Yuemei; Morton, James C.; Ramirez, Jose Luis; Souza-Neto, Jayme A.; Dimopoulos, George.

In: Insect Biochemistry and Molecular Biology, Vol. 42, No. 2, 02.2012, p. 126-132.

Research output: Contribution to journalArticle

@article{870d38300d90402faf6b6e9026497250,
title = "The entomopathogenic fungus Beauveria bassiana activate toll and JAK-STAT pathway-controlled effector genes and anti-dengue activity in Aedes aegypti",
abstract = "Here we investigated the effect of Beauveria bassiana infection on the survival of Aedes aegypti mosquitoes and the modulation of their susceptibility to dengue virus infection. Application of B.bassiana caused a reduction in the life span of A.aegypti and hindered dengue virus replication in the mosquito midgut. Fungus infection induced the expression a variety anti-microbial and dengue virus restriction factor genes. Transient reverse genetic analyses showed that the JAK-STAT pathway is implicated anti-fungal defense of Aedes mosquitoes. Our data suggest that this B.bassiana-mediated anti-dengue activity is likely to be at least partly indirectly mediated through the activation of the mosquito's anti-dengue Toll and JAK-STAT pathways.",
keywords = "Aedes aegypti, Beauveria bassiana, Dengue virus, Innate immunity",
author = "Yuemei Dong and Morton, {James C.} and Ramirez, {Jose Luis} and Souza-Neto, {Jayme A.} and George Dimopoulos",
year = "2012",
month = "2",
doi = "10.1016/j.ibmb.2011.11.005",
language = "English (US)",
volume = "42",
pages = "126--132",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - The entomopathogenic fungus Beauveria bassiana activate toll and JAK-STAT pathway-controlled effector genes and anti-dengue activity in Aedes aegypti

AU - Dong, Yuemei

AU - Morton, James C.

AU - Ramirez, Jose Luis

AU - Souza-Neto, Jayme A.

AU - Dimopoulos, George

PY - 2012/2

Y1 - 2012/2

N2 - Here we investigated the effect of Beauveria bassiana infection on the survival of Aedes aegypti mosquitoes and the modulation of their susceptibility to dengue virus infection. Application of B.bassiana caused a reduction in the life span of A.aegypti and hindered dengue virus replication in the mosquito midgut. Fungus infection induced the expression a variety anti-microbial and dengue virus restriction factor genes. Transient reverse genetic analyses showed that the JAK-STAT pathway is implicated anti-fungal defense of Aedes mosquitoes. Our data suggest that this B.bassiana-mediated anti-dengue activity is likely to be at least partly indirectly mediated through the activation of the mosquito's anti-dengue Toll and JAK-STAT pathways.

AB - Here we investigated the effect of Beauveria bassiana infection on the survival of Aedes aegypti mosquitoes and the modulation of their susceptibility to dengue virus infection. Application of B.bassiana caused a reduction in the life span of A.aegypti and hindered dengue virus replication in the mosquito midgut. Fungus infection induced the expression a variety anti-microbial and dengue virus restriction factor genes. Transient reverse genetic analyses showed that the JAK-STAT pathway is implicated anti-fungal defense of Aedes mosquitoes. Our data suggest that this B.bassiana-mediated anti-dengue activity is likely to be at least partly indirectly mediated through the activation of the mosquito's anti-dengue Toll and JAK-STAT pathways.

KW - Aedes aegypti

KW - Beauveria bassiana

KW - Dengue virus

KW - Innate immunity

UR - http://www.scopus.com/inward/record.url?scp=84855568563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84855568563&partnerID=8YFLogxK

U2 - 10.1016/j.ibmb.2011.11.005

DO - 10.1016/j.ibmb.2011.11.005

M3 - Article

C2 - 22198333

AN - SCOPUS:84855568563

VL - 42

SP - 126

EP - 132

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 2

ER -