The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

Ravindra A. De Silva; Dananjaya K. Appulage; Halina Pietraszkiewicz; Kevin R. Bobbitt; Joe Media; Jia Jiu Shaw; Fred A. Valeriote; Peter R. Andreana

doi:10.1007/s00262-012-1205-9

The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

Ravindra A. De Silva, Dananjaya K. Appulage, Halina Pietraszkiewicz, Kevin R. Bobbitt, Joe Media, Jia Jiu Shaw, Fred A. Valeriote, Peter R. Andreana

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; αNAc-ONH₂) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.

Original language	English (US)
Pages (from-to)	581-585
Number of pages	5
Journal	Cancer Immunology, Immunotherapy
Volume	61
Issue number	4
DOIs	https://doi.org/10.1007/s00262-012-1205-9
State	Published - Apr 2012
Externally published	Yes

Keywords

Antigen
Carbohydrates
Flow cytometry
Interleukin 17
Tumor
Vaccine

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Oncology
Cancer Research

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10.1007/s00262-012-1205-9

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@article{995064b5682d4e3990397240d78a66c6,

title = "The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17",

abstract = "The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; αNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.",

keywords = "Antigen, Carbohydrates, Flow cytometry, Interleukin 17, Tumor, Vaccine",

author = "{De Silva}, {Ravindra A.} and Appulage, {Dananjaya K.} and Halina Pietraszkiewicz and Bobbitt, {Kevin R.} and Joe Media and Shaw, {Jia Jiu} and Valeriote, {Fred A.} and Andreana, {Peter R.}",

note = "Funding Information: Acknowledgments Authors thank Dr. Mary Kay Pflum for Jurkat and JurkatTAg cell lines. P.R.A acknowledges Wayne State University for financial support and the National Institutes of Health/ National Cancer Institute for an R01 CA 156661 award.",

year = "2012",

month = apr,

doi = "10.1007/s00262-012-1205-9",

language = "English (US)",

volume = "61",

pages = "581--585",

journal = "Cancer Immunology, Immunotherapy",

issn = "0340-7004",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "4",

}

TY - JOUR

T1 - The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

AU - De Silva, Ravindra A.

AU - Appulage, Dananjaya K.

AU - Pietraszkiewicz, Halina

AU - Bobbitt, Kevin R.

AU - Media, Joe

AU - Shaw, Jia Jiu

AU - Valeriote, Fred A.

AU - Andreana, Peter R.

N1 - Funding Information: Acknowledgments Authors thank Dr. Mary Kay Pflum for Jurkat and JurkatTAg cell lines. P.R.A acknowledges Wayne State University for financial support and the National Institutes of Health/ National Cancer Institute for an R01 CA 156661 award.

PY - 2012/4

Y1 - 2012/4

N2 - The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; αNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.

AB - The tumor-associated carbohydrate antigen/hapten Thomsen-nouveau (Tn; αNAc-ONH2) was conjugated to a zwitterionic capsular polysaccharide, PS A1, from commensal anaerobe Bacteroides fragilis ATCC 25285/NCTC 9343 for the development of an entirely carbohydrate cancer vaccine construct and probed for immunogenicity. This communication discloses that murine anti-Tn IgG3 antibodies both bind to and recognize human tumor cells that display the Tn hapten. Furthermore, the sera from immunization of mice with Tn-PS A1 contain cytokine interleukin 17 (IL-17A), which is known to possess anti-tumor function and represents a striking difference to an IL-2, and IL-6 profile obtained with anti-PS A1 sera.

KW - Antigen

KW - Carbohydrates

KW - Flow cytometry

KW - Interleukin 17

KW - Tumor

KW - Vaccine

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JO - Cancer Immunology, Immunotherapy

JF - Cancer Immunology, Immunotherapy

IS - 4

ER -

The entirely carbohydrate immunogen Tn-PS A1 induces a cancer cell selective immune response and cytokine IL-17

Abstract

Keywords

ASJC Scopus subject areas

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