The emerging role of insertions and deletions in protein engineering

David R Shortle, John Sondek

Research output: Contribution to journalArticle

Abstract

Most attempts to engineer the properties of proteins have employed single or multiple substitution mutations, which typically produce minor changes in structure. Recent structural and stability studies of insertion and deletion mutants clearly indicate that relatively large structural perturbations can be induced by altering the spacing of residues along the polypeptide backbone, often without major losses in protein stability. Although their effects are difficult to anticipate, insertions and deletions provide important new tools for altering protein structures in directions not achievable with substitutions alone.

Original languageEnglish (US)
Pages (from-to)387-393
Number of pages7
JournalCurrent Opinion in Biotechnology
Volume6
Issue number4
DOIs
StatePublished - 1995

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Protein Engineering
Proteins
Protein Stability
Substitution reactions
Polypeptides
Peptides
Mutation
Engineers
Direction compound

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The emerging role of insertions and deletions in protein engineering. / Shortle, David R; Sondek, John.

In: Current Opinion in Biotechnology, Vol. 6, No. 4, 1995, p. 387-393.

Research output: Contribution to journalArticle

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