The effects of several croton oil constituents on two types of DNA repair and cyclic nucleotide levels in mammalian cells in vitro

James E. Trosko, James D. Yager, G. T. Bowden, Fred R. Butcher

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of the present study was to determine the effects of two potent tumor-promoting agents on two DNA repair mechanisms and cyclic nucleotide levels in mammalian cells. Human amnion (AV3) cells were treated with low dose levels of either UV or N-acetoxy-acetylaminofluorene. Subsequently, DNA excision repair as measured by unscheduled DNA synthesis was followed in the absence or presence of non-toxic levels of either 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol-12,13-dibenzoate (PDB), both potent tumor promoters, or phorbol, a non-promoter. Neither of these compounds inhibited DNA repair synthesis occurring in response to low doses of the carcinogenic agents. In addition, TPA did not inhibit "post-replication repair" in response to UV irradiation of growing Chinese hamster (V79-4) cells. However, both TPA and PDB did cause rapid dramatic increases in cyclic guanosine.

Original languageEnglish (US)
Pages (from-to)191-205
Number of pages15
JournalChemico-Biological Interactions
Volume11
Issue number3
DOIs
StatePublished - Sep 1975
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology

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