TY - JOUR
T1 - The effects of several croton oil constituents on two types of DNA repair and cyclic nucleotide levels in mammalian cells in vitro
AU - Trosko, James E.
AU - Yager, James D.
AU - Bowden, G. T.
AU - Butcher, Fred R.
N1 - Funding Information:
+ Work was performed whde J.E.T. was a Career Development Awardee of the Public Health Services (1 K 4-CA 29,085-Ol) on leave to the McArdle Laboratory from Michii State University. Research was supported by a grant (CA 13048-01) from the National Cancer Institute to J.E.T.. by a Departmental Grant CA-07175 to the McArdle Laboratory and grant lN-45N from the American Cancer Society to F.R.B. * To whom requests for reprints should be sent at the Department of Human Development, Michii State University, E. Lansing, Mich. 48823 (U.S.A.). b Present address: Department of Biological Sciences, Dartmouth College, Hanover, NH. 03755 (U.S.A.). c Present address: Institut fiir Biochemie, Deutsches Krehsforschungszentr, Kirschnerstrasse6 , Germany. Abbreviations: AAF, 2-acetylaminofluorene; JV-acetoxy-AAF, N-acetoxy acetylaminofluorene; CAMP, cyclic adenosine monophosphate; cGMP, cyclic guanosine monophosphate; DMSO,d imethyl sulfoxide; MEM, Eagle’sm inimal medium; PDB, phorbol-lS13dibenzoatezoareP; HlTdR, 13H-Me4-thymidine; TPA, 12-0-tetradecanoylphorbol-13-acetate.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1975/9
Y1 - 1975/9
N2 - The purpose of the present study was to determine the effects of two potent tumor-promoting agents on two DNA repair mechanisms and cyclic nucleotide levels in mammalian cells. Human amnion (AV3) cells were treated with low dose levels of either UV or N-acetoxy-acetylaminofluorene. Subsequently, DNA excision repair as measured by unscheduled DNA synthesis was followed in the absence or presence of non-toxic levels of either 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol-12,13-dibenzoate (PDB), both potent tumor promoters, or phorbol, a non-promoter. Neither of these compounds inhibited DNA repair synthesis occurring in response to low doses of the carcinogenic agents. In addition, TPA did not inhibit "post-replication repair" in response to UV irradiation of growing Chinese hamster (V79-4) cells. However, both TPA and PDB did cause rapid dramatic increases in cyclic guanosine.
AB - The purpose of the present study was to determine the effects of two potent tumor-promoting agents on two DNA repair mechanisms and cyclic nucleotide levels in mammalian cells. Human amnion (AV3) cells were treated with low dose levels of either UV or N-acetoxy-acetylaminofluorene. Subsequently, DNA excision repair as measured by unscheduled DNA synthesis was followed in the absence or presence of non-toxic levels of either 12-O-tetradecanoylphorbol-13-acetate (TPA), phorbol-12,13-dibenzoate (PDB), both potent tumor promoters, or phorbol, a non-promoter. Neither of these compounds inhibited DNA repair synthesis occurring in response to low doses of the carcinogenic agents. In addition, TPA did not inhibit "post-replication repair" in response to UV irradiation of growing Chinese hamster (V79-4) cells. However, both TPA and PDB did cause rapid dramatic increases in cyclic guanosine.
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U2 - 10.1016/0009-2797(75)90099-X
DO - 10.1016/0009-2797(75)90099-X
M3 - Article
C2 - 168978
AN - SCOPUS:0016791287
SN - 0009-2797
VL - 11
SP - 191
EP - 205
JO - Chemico-Biological Interactions
JF - Chemico-Biological Interactions
IS - 3
ER -