The effects of renal transplantation on peripheral blood dentritic cells

Karl L. Womer, Ruihua Peng, Pamela R. Patton, Matthew R. Murawski, Michael Bucci, Ayesha Kaleem, Jesse Schold, Phillip A. Efron, Alan W. Hemming, T. R. Srinivas, Herwig Ulf Meier-Kriesche, Bruce Kaplan, Michael J. Clare-Salzler

Research output: Contribution to journalArticle

Abstract

Recent advances allow accurate quantification of peripheral blood (PB) myeloid and plasmacytoid dendritic cell (DC) populations (mDC and pDC, respectively), although the response to renal transplantation (RT) remains unknown. Using flow cytometry, PBDC levels were quantified in patients with end stage renal disease (ESRD) undergoing RT. PBDC levels were significantly reduced in ESRD patients pre-RT compared to healthy controls, with further reduction noted immediately following a hemodialysis session. RT resulted in a dramatic decrease in both subsets, with a greater reduction of pDC levels. Both subset levels were significantly lower than in control patients undergoing abdominal surgery without RT. Subgroup analysis revealed significantly greater mDC reduction in RT recipients receiving anti-lymphocyte therapy, with preferential binding of antibody preparation to this subset. Samples from later time points revealed a gradual return of PBDC levels back to pre-transplant values concurrent with overall reduction of immunosuppression (IS). Finally, PBDC levels were significantly reduced in patients with BK virus nephropathy compared to recipients with stable graft function, despite lower overall IS. Our findings suggest that PBDC levels reflect the degree of IS in renal allograft recipients. Furthermore, PBDC monitoring may represent a novel strategy to predict important outcomes such as acute rejection, long-term graft loss and infectious complications.

Original languageEnglish (US)
Pages (from-to)659-667
Number of pages9
JournalClinical Transplantation
Volume19
Issue number5
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Kidney Transplantation
Blood Cells
Immunosuppression
Transplants
Chronic Kidney Failure
BK Virus
Dendritic Cells
Allografts
Renal Dialysis
Flow Cytometry
Lymphocytes
Kidney
Antibodies
Population

Keywords

  • Antihymocyte globulin
  • BK virus
  • Dentritic cells
  • Hemodialysis
  • Immunosuppression
  • Renal transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Womer, K. L., Peng, R., Patton, P. R., Murawski, M. R., Bucci, M., Kaleem, A., ... Clare-Salzler, M. J. (2005). The effects of renal transplantation on peripheral blood dentritic cells. Clinical Transplantation, 19(5), 659-667. https://doi.org/10.1111/j.1399-0012.2005.00405.x

The effects of renal transplantation on peripheral blood dentritic cells. / Womer, Karl L.; Peng, Ruihua; Patton, Pamela R.; Murawski, Matthew R.; Bucci, Michael; Kaleem, Ayesha; Schold, Jesse; Efron, Phillip A.; Hemming, Alan W.; Srinivas, T. R.; Meier-Kriesche, Herwig Ulf; Kaplan, Bruce; Clare-Salzler, Michael J.

In: Clinical Transplantation, Vol. 19, No. 5, 10.2005, p. 659-667.

Research output: Contribution to journalArticle

Womer, KL, Peng, R, Patton, PR, Murawski, MR, Bucci, M, Kaleem, A, Schold, J, Efron, PA, Hemming, AW, Srinivas, TR, Meier-Kriesche, HU, Kaplan, B & Clare-Salzler, MJ 2005, 'The effects of renal transplantation on peripheral blood dentritic cells', Clinical Transplantation, vol. 19, no. 5, pp. 659-667. https://doi.org/10.1111/j.1399-0012.2005.00405.x
Womer, Karl L. ; Peng, Ruihua ; Patton, Pamela R. ; Murawski, Matthew R. ; Bucci, Michael ; Kaleem, Ayesha ; Schold, Jesse ; Efron, Phillip A. ; Hemming, Alan W. ; Srinivas, T. R. ; Meier-Kriesche, Herwig Ulf ; Kaplan, Bruce ; Clare-Salzler, Michael J. / The effects of renal transplantation on peripheral blood dentritic cells. In: Clinical Transplantation. 2005 ; Vol. 19, No. 5. pp. 659-667.
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