The effects of renal transplantation on circulating precursor dendritic cells

K. L. Womer, R. Peng, P. R. Patton, A. Kaleem, M. Bucci, M. R. Murawski, J. Schold, P. A. Efron, A. W. Hemming, T. R. Srinivas, H. U. Meier-Kriesche, B. Kaplan, M. J. Clare-Salzler

Research output: Contribution to journalArticle

Abstract

Background. Dendritic cells (DCs) are potent antigen-presenting cells that induce and regulate immune responses. Recent advances allow accurate quantification of peripheral blood (PB) myeloid and plasmacytoid DC populations (mDC and pDC, respectively), although the response to renal transplantation (RT) remains unknown. Methods. Using flow cytometry, PBDC levels were quantified in patients with end-stage renal disease (ESRD) undergoing renal transplantation. Results. PBDC levels were significantly reduced in ESRD patients pretransplantation compared to healthy controls, with further reduction noted immediately following a hemodialysis session. RT resulted in a dramatic decrease in both subsets, with a greater reduction of pDC levels. Both subset levels were significantly lower than in control patients undergoing abdominal surgery without RT. Subgroup analysis revealed significantly greater mDC reduction in RT recipients receiving antilymphocyte therapy, with preferential binding of antibody preparation to this subset. Samples from later time points revealed a gradual return of PBDC levels back to pretransplant values concurrent with overall reduction of immunosuppression. Finally, PBDC levels were significantly reduced in patients with BK virus nephropathy compared to recipients with stable graft function, despite lower overall immunosuppression. Conclusions. Our findings suggest that PBDC levels may reflect the degree of immunosuppression in renal allograft recipients. Furthermore, PBDC monitoring may represent a novel strategy to predict important outcomes such as acute rejection, long-term graft loss, and infectious complications.

Original languageEnglish (US)
Pages (from-to)3-6
Number of pages4
JournalTransplantation Proceedings
Volume37
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

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Kidney Transplantation
Dendritic Cells
Immunosuppression
Chronic Kidney Failure
BK Virus
Transplants
Antigen-Presenting Cells
Allografts
Renal Dialysis
Flow Cytometry
Kidney
Antibodies
Population
Therapeutics

ASJC Scopus subject areas

  • Surgery
  • Transplantation

Cite this

Womer, K. L., Peng, R., Patton, P. R., Kaleem, A., Bucci, M., Murawski, M. R., ... Clare-Salzler, M. J. (2005). The effects of renal transplantation on circulating precursor dendritic cells. Transplantation Proceedings, 37(1), 3-6. https://doi.org/10.1016/j.transproceed.2004.11.076

The effects of renal transplantation on circulating precursor dendritic cells. / Womer, K. L.; Peng, R.; Patton, P. R.; Kaleem, A.; Bucci, M.; Murawski, M. R.; Schold, J.; Efron, P. A.; Hemming, A. W.; Srinivas, T. R.; Meier-Kriesche, H. U.; Kaplan, B.; Clare-Salzler, M. J.

In: Transplantation Proceedings, Vol. 37, No. 1, 01.2005, p. 3-6.

Research output: Contribution to journalArticle

Womer, KL, Peng, R, Patton, PR, Kaleem, A, Bucci, M, Murawski, MR, Schold, J, Efron, PA, Hemming, AW, Srinivas, TR, Meier-Kriesche, HU, Kaplan, B & Clare-Salzler, MJ 2005, 'The effects of renal transplantation on circulating precursor dendritic cells', Transplantation Proceedings, vol. 37, no. 1, pp. 3-6. https://doi.org/10.1016/j.transproceed.2004.11.076
Womer, K. L. ; Peng, R. ; Patton, P. R. ; Kaleem, A. ; Bucci, M. ; Murawski, M. R. ; Schold, J. ; Efron, P. A. ; Hemming, A. W. ; Srinivas, T. R. ; Meier-Kriesche, H. U. ; Kaplan, B. ; Clare-Salzler, M. J. / The effects of renal transplantation on circulating precursor dendritic cells. In: Transplantation Proceedings. 2005 ; Vol. 37, No. 1. pp. 3-6.
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