The effects of "oxygen radicals" generated in the medium on lenses in organ culture: Inhibition of damage by chelated iron

J. S. Zigler, H. M. Jernigan, D. Garland, V. N. Reddy

Research output: Contribution to journalArticle

Abstract

Rat lenses in organ culture were exposed to activated species of oxygen generated in the culture medium either by xanthine oxidase and hypoxanthine or by riboflavin and visible light, two systems which have been shown to produce superoxide and H2O2. In each case there was marked damage to carrier-mediated transport systems of the lens. Under standard culture conditions this damage was strongly inhibited by catalase, but not by superoxide dismutase (SOD). By the addition to the medium of chelated iron, hydroxyl radicals were produced in a Fenton reaction with a concomitant decrease in H2O2 levels. With both oxygen radical-generating systems, the addition of chelated iron strongly inhibited lens damage. This inhibitory effect could be reversed by the addition of SOD with the chelated iron. Under such conditions SOD converts superoxide anion to H2O2, thereby preventing reduction of the chelated iron and thus stopping the generation of hydroxyl radicals. Increased lens damage following addition of SOD to the iron-containing systems correlated with higher H2O2 concentrations, and was inhibited by catalase. These findings suggest that, when generated in the fluids surrounding the lens, H2O2 poses a much greater oxidative stress for the lens than do the superoxide or hydroxyl free radicals.

Original languageEnglish (US)
Pages (from-to)163-172
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume241
Issue number1
DOIs
StatePublished - Aug 15 1985
Externally publishedYes

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Organ Culture Techniques
Lenses
Reactive Oxygen Species
Iron
Superoxide Dismutase
Superoxides
Hydroxyl Radical
Catalase
Oxidative stress
Carrier transport
Riboflavin
Xanthine Oxidase
Free Radicals
Culture Media
Rats
Oxidative Stress
Oxygen
Light
Fluids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The effects of "oxygen radicals" generated in the medium on lenses in organ culture : Inhibition of damage by chelated iron. / Zigler, J. S.; Jernigan, H. M.; Garland, D.; Reddy, V. N.

In: Archives of Biochemistry and Biophysics, Vol. 241, No. 1, 15.08.1985, p. 163-172.

Research output: Contribution to journalArticle

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