TY - JOUR
T1 - The effects of magnetic resonance imaging-guided high-intensity focused ultrasound ablation on human cadaver breast tissue
AU - Merckel, Laura G.
AU - Deckers, Roel
AU - Baron, Paul
AU - Bleys, Ronald L.A.W.
AU - Van Diest, Paul J.
AU - Moonen, Chrit T.W.
AU - Mali, Willem P.Th M.
AU - Van Den Bosch, Maurice A.A.J.
AU - Bartels, Lambertus W.
N1 - Funding Information:
We acknowledge Simon Plomp, Fiona van Zoomeren and Willem van Wolveren for their enthusiasm and help to obtain breast tissue for our experiments. We thank Baudouin Denis de Senneville and Mario Ries for helpful discussion. We greatly acknowledge the use of MeVisLab for the analyses of the deformation data. This research was supported by the Center for Translational Molecular Medicine (VOLTA) .
PY - 2013
Y1 - 2013
N2 - Magnetic Resonance Imaging-guided High-Intensity Focused Ultrasound (MR-HIFU) is a promising technique for non-invasive breast tumor ablation. The purpose of this study was to investigate the effects of HIFU ablation and thermal exposure on ex vivo human breast tissue. HIFU ablations were performed in three unembalmed cadaveric breast specimens using a clinical MR-HIFU system. Sonications were performed in fibroglandular and adipose tissue. During HIFU ablation, time-resolved anatomical MR images were acquired to monitor macroscopic tissue changes. Furthermore, the breast tissue temperature was measured using a thermocouple to investigate heating and cooling under HIFU exposure. After HIFU ablation, breast tissue samples were excised and prepared for histopathological analysis. In addition, thermal exposure experiments were performed to distinguish between different levels of thermal damage using immunohistochemical staining. Irreversible macroscopic deformations up to 3.7 mm were observed upon HIFU ablation both in fibroglandular and in adipose tissue. No relationship was found between the sonication power or the maximum tissue temperature and the size of the deformations. Temperature measurements after HIFU ablation showed a slow decline in breast tissue temperature. Histopathological analysis of sonicated regions demonstrated ablated tissue and morphologically complete cell death. After thermal exposure, samples exposed to three different temperatures could readily be distinguished. In conclusion, the irreversible macroscopic tissue deformations in ex vivo human breast tissue observed during HIFU ablation suggest that it might be relevant to monitor tissue deformations during MR-HIFU treatments. Furthermore, the slow decrease in breast tissue temperature after HIFU ablation increases the risk of heat accumulation between successive sonications. Since cell death was inflicted after already 5 minutes at 75 C, MR-HIFU may find a place in non-invasive treatment of breast tumors.
AB - Magnetic Resonance Imaging-guided High-Intensity Focused Ultrasound (MR-HIFU) is a promising technique for non-invasive breast tumor ablation. The purpose of this study was to investigate the effects of HIFU ablation and thermal exposure on ex vivo human breast tissue. HIFU ablations were performed in three unembalmed cadaveric breast specimens using a clinical MR-HIFU system. Sonications were performed in fibroglandular and adipose tissue. During HIFU ablation, time-resolved anatomical MR images were acquired to monitor macroscopic tissue changes. Furthermore, the breast tissue temperature was measured using a thermocouple to investigate heating and cooling under HIFU exposure. After HIFU ablation, breast tissue samples were excised and prepared for histopathological analysis. In addition, thermal exposure experiments were performed to distinguish between different levels of thermal damage using immunohistochemical staining. Irreversible macroscopic deformations up to 3.7 mm were observed upon HIFU ablation both in fibroglandular and in adipose tissue. No relationship was found between the sonication power or the maximum tissue temperature and the size of the deformations. Temperature measurements after HIFU ablation showed a slow decline in breast tissue temperature. Histopathological analysis of sonicated regions demonstrated ablated tissue and morphologically complete cell death. After thermal exposure, samples exposed to three different temperatures could readily be distinguished. In conclusion, the irreversible macroscopic tissue deformations in ex vivo human breast tissue observed during HIFU ablation suggest that it might be relevant to monitor tissue deformations during MR-HIFU treatments. Furthermore, the slow decrease in breast tissue temperature after HIFU ablation increases the risk of heat accumulation between successive sonications. Since cell death was inflicted after already 5 minutes at 75 C, MR-HIFU may find a place in non-invasive treatment of breast tumors.
KW - Breast tissue
KW - High-Intensity Focused Ultrasound
KW - Magnetic Resonance Imaging
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U2 - 10.1016/j.ejphar.2012.11.070
DO - 10.1016/j.ejphar.2012.11.070
M3 - Review article
C2 - 23583321
AN - SCOPUS:84885958673
SN - 0014-2999
VL - 717
SP - 21
EP - 30
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-3
ER -