The effects of freeze-thaw on β-trace protein and β2-microglobulin assays after long-term sample storage

Stephen P. Juraschek, Josef Coresh, Lesley A. Inker, Gregory P. Rynders, John H. Eckfeldt, Elizabeth Selvin

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To evaluate the effect of a freeze-thaw cycle on β-trace protein (βTP) and β2-microglobulin (β2M). Design and methods: We compared βTP and β2M concentrations before and after a single freeze-thaw cycle in long-term stored samples from 172 participants of the Third National Health and Nutrition Examination Survey (NHANES III). Results: Measurements of βTP and β2M before and after freeze-thaw were highly correlated with Spearman's coefficients of 0.90 and 0.99, respectively. Serum concentrations of βTP were slightly lower after freeze-thaw (- 0.05. mg/L, P= 0.006). Measurements of β2M did not differ before and after freeze-thaw (P= 0.35). Conclusions: βTP and β2M measurements were robust to a single freeze-thaw cycle, although β2M appeared more stable than βTP. These results have implications for future studies of these biomarkers.

Original languageEnglish (US)
Pages (from-to)694-696
Number of pages3
JournalClinical Biochemistry
Volume45
Issue number9
DOIs
StatePublished - Jun 1 2012

Keywords

  • Freeze-thaw
  • Kidney filtration marker
  • National Health and Nutrition Examination Survey
  • β-trace protein (βTP)
  • β2-microglobulin

ASJC Scopus subject areas

  • Clinical Biochemistry

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