The effects of donor-specific blood transfusion enhancement of rat renal allografts on host NK cell responses

Barbara Wasowska, David N. Howell, William M. Baldwin, Fred Sanfilippo

Research output: Contribution to journalArticlepeer-review

Abstract

Donor-specific blood transfusion (DSBT) given 1-2 weeks prior to transplantation prolongs the survival of fully allogeneic ACI (RT1") renal allografts in PVG (RT1C) recipients from 6-8 days to >100 days. We have previously demonstrated that ACI kidneys transplanted to autologous blood transfusion (ABT)- or DSBT-pre-treated PVG recipients stimulated an increase in CD8+(OX8+) cells in the peripheral blood by 6 days after transplantation. To determine whether this increase represents a general expansion of the entire CD8+ population or only a subpopulation of CD8+ cells, subset analysis was performed on peripheral blood lymphocytes depleted of cells reactive with monoclonal antibodies against rat a/9 T cell receptor (TCR), CD8, or NK cells (R7.3, 0X8, or 3.2.3, respectively). Phenotypic studies of PBL depleted of CD8+ cells demonstrated that all 3.2.3+ NK cells coexpressed CD8; depletion of 3.2.3* PBL revealed a secondsubpopulation of CD8+3.2.3~ cells comprised predominantly of a/8TCR+ T cells. In naive PVG rats the prevelence of these two CD8 subpopulations was approximately equal. Both ABT- and DSBT-pretreated renal allograft recipients demonstrated a significant and equivalent expansion of the CD8+ cell subpopulation that coexpresses the 3.2.3 NK marker. In contrast, the second subpopulation of CD8+3.2.3- cells did not change significantly after allografting. There were also no differences between DSBT and ABT pretreated rats in activity of PBL against the NK targets YAC-1 and Doxie at 6 days after renal transplantation, though the level of activity was modestly increased compared with naive controls. These findings indicate that renal transplantation in the rat is associated with a significant increase in PBL with the NK phenotype (CD8+3.2.3+) and a modest increase of NK activity, but that DSBT enhancement does not affect this NK cell response.

Original languageEnglish (US)
Pages (from-to)136-142
Number of pages7
JournalTransplantation
Volume54
Issue number1
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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