Abstract
Donor-specific blood transfusion (DSBT) given 1-2 weeks prior to transplantation prolongs the survival of fully allogeneic ACI (RT1") renal allografts in PVG (RT1C) recipients from 6-8 days to >100 days. We have previously demonstrated that ACI kidneys transplanted to autologous blood transfusion (ABT)- or DSBT-pre-treated PVG recipients stimulated an increase in CD8+(OX8+) cells in the peripheral blood by 6 days after transplantation. To determine whether this increase represents a general expansion of the entire CD8+ population or only a subpopulation of CD8+ cells, subset analysis was performed on peripheral blood lymphocytes depleted of cells reactive with monoclonal antibodies against rat a/9 T cell receptor (TCR), CD8, or NK cells (R7.3, 0X8, or 3.2.3, respectively). Phenotypic studies of PBL depleted of CD8+ cells demonstrated that all 3.2.3+ NK cells coexpressed CD8; depletion of 3.2.3* PBL revealed a secondsubpopulation of CD8+3.2.3~ cells comprised predominantly of a/8TCR+ T cells. In naive PVG rats the prevelence of these two CD8 subpopulations was approximately equal. Both ABT- and DSBT-pretreated renal allograft recipients demonstrated a significant and equivalent expansion of the CD8+ cell subpopulation that coexpresses the 3.2.3 NK marker. In contrast, the second subpopulation of CD8+3.2.3- cells did not change significantly after allografting. There were also no differences between DSBT and ABT pretreated rats in activity of PBL against the NK targets YAC-1 and Doxie at 6 days after renal transplantation, though the level of activity was modestly increased compared with naive controls. These findings indicate that renal transplantation in the rat is associated with a significant increase in PBL with the NK phenotype (CD8+3.2.3+) and a modest increase of NK activity, but that DSBT enhancement does not affect this NK cell response.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 136-142 |
| Number of pages | 7 |
| Journal | Transplantation |
| Volume | 54 |
| Issue number | 1 |
| State | Published - 1992 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Transplantation
- Immunology
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The effects of donor-specific blood transfusion enhancement of rat renal allografts on host NK cell responses. / Wasowska, Barbara; Howell, David N.; Baldwin, William M.; Sanfilippo, Fred.
In: Transplantation, Vol. 54, No. 1, 1992, p. 136-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The effects of donor-specific blood transfusion enhancement of rat renal allografts on host NK cell responses
AU - Wasowska, Barbara
AU - Howell, David N.
AU - Baldwin, William M.
AU - Sanfilippo, Fred
PY - 1992
Y1 - 1992
N2 - Donor-specific blood transfusion (DSBT) given 1-2 weeks prior to transplantation prolongs the survival of fully allogeneic ACI (RT1") renal allografts in PVG (RT1C) recipients from 6-8 days to >100 days. We have previously demonstrated that ACI kidneys transplanted to autologous blood transfusion (ABT)- or DSBT-pre-treated PVG recipients stimulated an increase in CD8+(OX8+) cells in the peripheral blood by 6 days after transplantation. To determine whether this increase represents a general expansion of the entire CD8+ population or only a subpopulation of CD8+ cells, subset analysis was performed on peripheral blood lymphocytes depleted of cells reactive with monoclonal antibodies against rat a/9 T cell receptor (TCR), CD8, or NK cells (R7.3, 0X8, or 3.2.3, respectively). Phenotypic studies of PBL depleted of CD8+ cells demonstrated that all 3.2.3+ NK cells coexpressed CD8; depletion of 3.2.3* PBL revealed a secondsubpopulation of CD8+3.2.3~ cells comprised predominantly of a/8TCR+ T cells. In naive PVG rats the prevelence of these two CD8 subpopulations was approximately equal. Both ABT- and DSBT-pretreated renal allograft recipients demonstrated a significant and equivalent expansion of the CD8+ cell subpopulation that coexpresses the 3.2.3 NK marker. In contrast, the second subpopulation of CD8+3.2.3- cells did not change significantly after allografting. There were also no differences between DSBT and ABT pretreated rats in activity of PBL against the NK targets YAC-1 and Doxie at 6 days after renal transplantation, though the level of activity was modestly increased compared with naive controls. These findings indicate that renal transplantation in the rat is associated with a significant increase in PBL with the NK phenotype (CD8+3.2.3+) and a modest increase of NK activity, but that DSBT enhancement does not affect this NK cell response.
AB - Donor-specific blood transfusion (DSBT) given 1-2 weeks prior to transplantation prolongs the survival of fully allogeneic ACI (RT1") renal allografts in PVG (RT1C) recipients from 6-8 days to >100 days. We have previously demonstrated that ACI kidneys transplanted to autologous blood transfusion (ABT)- or DSBT-pre-treated PVG recipients stimulated an increase in CD8+(OX8+) cells in the peripheral blood by 6 days after transplantation. To determine whether this increase represents a general expansion of the entire CD8+ population or only a subpopulation of CD8+ cells, subset analysis was performed on peripheral blood lymphocytes depleted of cells reactive with monoclonal antibodies against rat a/9 T cell receptor (TCR), CD8, or NK cells (R7.3, 0X8, or 3.2.3, respectively). Phenotypic studies of PBL depleted of CD8+ cells demonstrated that all 3.2.3+ NK cells coexpressed CD8; depletion of 3.2.3* PBL revealed a secondsubpopulation of CD8+3.2.3~ cells comprised predominantly of a/8TCR+ T cells. In naive PVG rats the prevelence of these two CD8 subpopulations was approximately equal. Both ABT- and DSBT-pretreated renal allograft recipients demonstrated a significant and equivalent expansion of the CD8+ cell subpopulation that coexpresses the 3.2.3 NK marker. In contrast, the second subpopulation of CD8+3.2.3- cells did not change significantly after allografting. There were also no differences between DSBT and ABT pretreated rats in activity of PBL against the NK targets YAC-1 and Doxie at 6 days after renal transplantation, though the level of activity was modestly increased compared with naive controls. These findings indicate that renal transplantation in the rat is associated with a significant increase in PBL with the NK phenotype (CD8+3.2.3+) and a modest increase of NK activity, but that DSBT enhancement does not affect this NK cell response.
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M3 - Article
VL - 54
SP - 136
EP - 142
JO - Transplantation
JF - Transplantation
SN - 0041-1337
IS - 1
ER -