The effects of demineralized bone matrix and direct current on an “in vivo” culture of bone marrow cells

Zachary B. Friedenberg, Carl T. Brighton, James D. Michelson, John Bednar, Richard Schmidt, Thomas Brockmeyer

Research output: Contribution to journalArticlepeer-review

Abstract

Bone marrow cells (BMCs) from rabbit femora and tibiae were grown in diffusion chambers implanted in rabbit muscle. At 42 days 80% of the BMC chambers exhibited cartilage formation within them. Demineralized bone matrix added to the marrow cell suspension in the chamber accelerated the appearance and increased the number of chambers with cartilage. Mineralization of the cartilage also occurred earlier in the chambers with bone matrix. In a second experiment, a 5‐μA direct current cathode in the bone marrow chamber increased the number of chambers containing cartilage from 50 to 80% at day 25. Mineralization also occurred earlier in the chambers with direct current.

Original languageEnglish (US)
Pages (from-to)22-27
Number of pages6
JournalJournal of Orthopaedic Research
Volume7
Issue number1
DOIs
StatePublished - Jan 1989

Keywords

  • Demineralized bone matrix
  • Direct current in tissue
  • Millipore diffusion chamber

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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