The effects of demineralized bone matrix and direct current on an “in vivo” culture of bone marrow cells

Zachary B. Friedenberg; Carl T. Brighton; James D. Michelson; John Bednar; Richard Schmidt; Thomas Brockmeyer

doi:10.1002/jor.1100070104

The effects of demineralized bone matrix and direct current on an “in vivo” culture of bone marrow cells

Zachary B. Friedenberg, Carl T. Brighton, James D. Michelson, John Bednar, Richard Schmidt, Thomas Brockmeyer

School of Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Bone marrow cells (BMCs) from rabbit femora and tibiae were grown in diffusion chambers implanted in rabbit muscle. At 42 days 80% of the BMC chambers exhibited cartilage formation within them. Demineralized bone matrix added to the marrow cell suspension in the chamber accelerated the appearance and increased the number of chambers with cartilage. Mineralization of the cartilage also occurred earlier in the chambers with bone matrix. In a second experiment, a 5‐μA direct current cathode in the bone marrow chamber increased the number of chambers containing cartilage from 50 to 80% at day 25. Mineralization also occurred earlier in the chambers with direct current.

Original language	English (US)
Pages (from-to)	22-27
Number of pages	6
Journal	Journal of Orthopaedic Research
Volume	7
Issue number	1
DOIs	https://doi.org/10.1002/jor.1100070104
State	Published - Jan 1989

Keywords

Demineralized bone matrix
Direct current in tissue
Millipore diffusion chamber

ASJC Scopus subject areas

Orthopedics and Sports Medicine

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10.1002/jor.1100070104

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title = "The effects of demineralized bone matrix and direct current on an “in vivo” culture of bone marrow cells",

abstract = "Bone marrow cells (BMCs) from rabbit femora and tibiae were grown in diffusion chambers implanted in rabbit muscle. At 42 days 80% of the BMC chambers exhibited cartilage formation within them. Demineralized bone matrix added to the marrow cell suspension in the chamber accelerated the appearance and increased the number of chambers with cartilage. Mineralization of the cartilage also occurred earlier in the chambers with bone matrix. In a second experiment, a 5‐μA direct current cathode in the bone marrow chamber increased the number of chambers containing cartilage from 50 to 80% at day 25. Mineralization also occurred earlier in the chambers with direct current.",

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author = "Friedenberg, {Zachary B.} and Brighton, {Carl T.} and Michelson, {James D.} and John Bednar and Richard Schmidt and Thomas Brockmeyer",

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T1 - The effects of demineralized bone matrix and direct current on an “in vivo” culture of bone marrow cells

AU - Friedenberg, Zachary B.

AU - Brighton, Carl T.

AU - Michelson, James D.

AU - Bednar, John

AU - Schmidt, Richard

AU - Brockmeyer, Thomas

PY - 1989/1

Y1 - 1989/1

N2 - Bone marrow cells (BMCs) from rabbit femora and tibiae were grown in diffusion chambers implanted in rabbit muscle. At 42 days 80% of the BMC chambers exhibited cartilage formation within them. Demineralized bone matrix added to the marrow cell suspension in the chamber accelerated the appearance and increased the number of chambers with cartilage. Mineralization of the cartilage also occurred earlier in the chambers with bone matrix. In a second experiment, a 5‐μA direct current cathode in the bone marrow chamber increased the number of chambers containing cartilage from 50 to 80% at day 25. Mineralization also occurred earlier in the chambers with direct current.

AB - Bone marrow cells (BMCs) from rabbit femora and tibiae were grown in diffusion chambers implanted in rabbit muscle. At 42 days 80% of the BMC chambers exhibited cartilage formation within them. Demineralized bone matrix added to the marrow cell suspension in the chamber accelerated the appearance and increased the number of chambers with cartilage. Mineralization of the cartilage also occurred earlier in the chambers with bone matrix. In a second experiment, a 5‐μA direct current cathode in the bone marrow chamber increased the number of chambers containing cartilage from 50 to 80% at day 25. Mineralization also occurred earlier in the chambers with direct current.

KW - Demineralized bone matrix

KW - Direct current in tissue

KW - Millipore diffusion chamber

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The effects of demineralized bone matrix and direct current on an “in vivo” culture of bone marrow cells

Abstract

Keywords

ASJC Scopus subject areas

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