The effects of ciprofloxacin on human chondrocytes in cell culture

M. A. Mont, S. K. Mathur, C. G. Frondoza, D. S. Hungerford

Research output: Contribution to journalArticle

Abstract

Ciprofloxacin is a highly potent antibacterial agent that is used extensively in bone and joint infections. Because of reports of potential chondro-toxicity in animals, the effects of this drug on cells derived from human cartilage were tested in liquid micromass and agarose gel cultures. An inhibition of cell proliferation as indicated by a decrease in [3H]-thymidine uptake and bromodeoxyuridine labeling at ciprofloxacin concentrations of 0.5 and 50 mg/l was found which corresponded to the therapeutic and toxic serum levels. There was no effect on proteoglycan synthesis as indicated by 35SO4 incorporation. Immunocytochemistry showed no changes in morphology or staining patterns for type-I procollagen, type-II collagen, keratan sulfate and unsulfated chondroitin. Because the amount of inhibition of DNA synthesis varied with different ciprofloxacin concentrations, this data suggests that this agent has a differential effect on newly differentiating cells and might be the basis for contraindication in pediatric patients.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalInfection
Volume24
Issue number2
DOIs
StatePublished - 1996

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Ciprofloxacin
Chondrocytes
Cell Culture Techniques
Chondroitin
Keratan Sulfate
Collagen Type II
Poisons
Proteoglycans
Bromodeoxyuridine
Sepharose
Thymidine
Cartilage
Collagen
Joints
Gels
Immunohistochemistry
Cell Proliferation
Pediatrics
Staining and Labeling
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology

Cite this

Mont, M. A., Mathur, S. K., Frondoza, C. G., & Hungerford, D. S. (1996). The effects of ciprofloxacin on human chondrocytes in cell culture. Infection, 24(2), 151-155. https://doi.org/10.1007/BF01713325

The effects of ciprofloxacin on human chondrocytes in cell culture. / Mont, M. A.; Mathur, S. K.; Frondoza, C. G.; Hungerford, D. S.

In: Infection, Vol. 24, No. 2, 1996, p. 151-155.

Research output: Contribution to journalArticle

Mont, MA, Mathur, SK, Frondoza, CG & Hungerford, DS 1996, 'The effects of ciprofloxacin on human chondrocytes in cell culture', Infection, vol. 24, no. 2, pp. 151-155. https://doi.org/10.1007/BF01713325
Mont, M. A. ; Mathur, S. K. ; Frondoza, C. G. ; Hungerford, D. S. / The effects of ciprofloxacin on human chondrocytes in cell culture. In: Infection. 1996 ; Vol. 24, No. 2. pp. 151-155.
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