The effect of vitamin A deficiency on hepatic, renal and pulmonary glutathione S-transferase activities in the rat

Z. H. Siddik, E. G. Mimnaugh, M. A. Trush, T. E. Gram

Research output: Contribution to journalArticlepeer-review

Abstract

Feeding male weanling rats on a vitamin A-deficient diet for 6 weeks resulted in significant increases (44-57%) in glutathione S-aryl-, S-aralkyl- S-alkyl- and S-epoxidetransferase activities in the liver cytosol. Only the S-aralkyl- (27%) and S-alkyltransferase (14%) activities were significantly increased in the kidney as a result of deficiency. There was no effect on any of the pulmonary glutathione S-transferase activities. The increases in hepatic transferase activities were due primarily to increases (25-96%) in the apparent V(max). There were no changes in the apparent K(m) of any of the four drug substrates employed. With 3,4-dichloronitrobenzene as the second substrate, the apparent K(m) for glutathione was increased by over 2-fold in vitamin A-deficient livers as compared with controls. The relationship between these results and enhanced susceptibility to chemical carcinogens in vitamin A deficiency is briefly discussed, and comparison is made between the effects of this nutritional state and pretreatment with drug inducers on the glutathione S-transferases.

Original languageEnglish (US)
Pages (from-to)889-893
Number of pages5
JournalBiochemical Journal
Volume188
Issue number3
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'The effect of vitamin A deficiency on hepatic, renal and pulmonary glutathione S-transferase activities in the rat'. Together they form a unique fingerprint.

Cite this