The effect of timing of pneumoperitoneum on the inflammatory response

S. L. Bachman, E. J. Hanly, J. I. Nwanko, J. Lamb, A. E. Herring, Michael Marohn, A. DeMaio, M. A. Talamini

Research output: Contribution to journalArticle

Abstract

Background: We examined the effects of an identical period of pneumoperitoneum applied at three different time points after lipopolysaccharide (LPS) challenge. Two different insufflation gases were also compared. Methods: Male rats (n = 70) were injected intravenously with 1 mg/kg of LPS (time 0). The time relationship between a 1.5-h period of insufflation and initial LPS stimulation was the experimental variable. All rats were killed 6 h after injection. CO2 and helium insufflation were investigated. Ten control rats received LPS only. Serum interleukin-6 (IL-6) levels were determined by enzyme-linked immunosorbent assay (ELISA). Hepatic expression of α2-macroglobulin, β-fibrinogen, and metallothionein were measured by Northern blot analysis. Statistical analysis was performed using one-way analysis of variance (ANOVA). Results: Expression of α2-macroglobulin mRNA was lower in CO2 groups compared to the control group (p <0.05 at time 120 and 270). β-Fibrinogen message was diminished in CO2 0 and 120 groups compared to control. Serum levels of IL-6 and expression of metallothionein mRNA did not show significant differences between groups. Conclusions: These findings suggest that CO2 pneumoperitoneum downregulates the inflammatory response to LPS challenge. Start time of CO2 insufflation does not appear to alter hepatic expression of acute phase genes. The mechanism of α2-macroglobulin downregulation does not appear to be due to IL-6.

Original languageEnglish (US)
Pages (from-to)1640-1644
Number of pages5
JournalSurgical Endoscopy and Other Interventional Techniques
Volume18
Issue number11
DOIs
StatePublished - Nov 2004

Fingerprint

Pneumoperitoneum
Insufflation
Lipopolysaccharides
Macroglobulins
Interleukin-6
Metallothionein
Fibrinogen
Down-Regulation
Messenger RNA
Helium
Liver
Serum
Northern Blotting
Analysis of Variance
Gases
Enzyme-Linked Immunosorbent Assay
Control Groups
Injections
Genes

Keywords

  • Acute-phase proteins
  • Endotoxemia
  • Inflammatory response
  • Laparoscopy
  • Lipopolysaccharide
  • Pneumoperitoneum

ASJC Scopus subject areas

  • Surgery

Cite this

Bachman, S. L., Hanly, E. J., Nwanko, J. I., Lamb, J., Herring, A. E., Marohn, M., ... Talamini, M. A. (2004). The effect of timing of pneumoperitoneum on the inflammatory response. Surgical Endoscopy and Other Interventional Techniques, 18(11), 1640-1644. https://doi.org/10.1007/s00464-003-8928-9

The effect of timing of pneumoperitoneum on the inflammatory response. / Bachman, S. L.; Hanly, E. J.; Nwanko, J. I.; Lamb, J.; Herring, A. E.; Marohn, Michael; DeMaio, A.; Talamini, M. A.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 18, No. 11, 11.2004, p. 1640-1644.

Research output: Contribution to journalArticle

Bachman, SL, Hanly, EJ, Nwanko, JI, Lamb, J, Herring, AE, Marohn, M, DeMaio, A & Talamini, MA 2004, 'The effect of timing of pneumoperitoneum on the inflammatory response', Surgical Endoscopy and Other Interventional Techniques, vol. 18, no. 11, pp. 1640-1644. https://doi.org/10.1007/s00464-003-8928-9
Bachman, S. L. ; Hanly, E. J. ; Nwanko, J. I. ; Lamb, J. ; Herring, A. E. ; Marohn, Michael ; DeMaio, A. ; Talamini, M. A. / The effect of timing of pneumoperitoneum on the inflammatory response. In: Surgical Endoscopy and Other Interventional Techniques. 2004 ; Vol. 18, No. 11. pp. 1640-1644.
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AU - Herring, A. E.

AU - Marohn, Michael

AU - DeMaio, A.

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N2 - Background: We examined the effects of an identical period of pneumoperitoneum applied at three different time points after lipopolysaccharide (LPS) challenge. Two different insufflation gases were also compared. Methods: Male rats (n = 70) were injected intravenously with 1 mg/kg of LPS (time 0). The time relationship between a 1.5-h period of insufflation and initial LPS stimulation was the experimental variable. All rats were killed 6 h after injection. CO2 and helium insufflation were investigated. Ten control rats received LPS only. Serum interleukin-6 (IL-6) levels were determined by enzyme-linked immunosorbent assay (ELISA). Hepatic expression of α2-macroglobulin, β-fibrinogen, and metallothionein were measured by Northern blot analysis. Statistical analysis was performed using one-way analysis of variance (ANOVA). Results: Expression of α2-macroglobulin mRNA was lower in CO2 groups compared to the control group (p <0.05 at time 120 and 270). β-Fibrinogen message was diminished in CO2 0 and 120 groups compared to control. Serum levels of IL-6 and expression of metallothionein mRNA did not show significant differences between groups. Conclusions: These findings suggest that CO2 pneumoperitoneum downregulates the inflammatory response to LPS challenge. Start time of CO2 insufflation does not appear to alter hepatic expression of acute phase genes. The mechanism of α2-macroglobulin downregulation does not appear to be due to IL-6.

AB - Background: We examined the effects of an identical period of pneumoperitoneum applied at three different time points after lipopolysaccharide (LPS) challenge. Two different insufflation gases were also compared. Methods: Male rats (n = 70) were injected intravenously with 1 mg/kg of LPS (time 0). The time relationship between a 1.5-h period of insufflation and initial LPS stimulation was the experimental variable. All rats were killed 6 h after injection. CO2 and helium insufflation were investigated. Ten control rats received LPS only. Serum interleukin-6 (IL-6) levels were determined by enzyme-linked immunosorbent assay (ELISA). Hepatic expression of α2-macroglobulin, β-fibrinogen, and metallothionein were measured by Northern blot analysis. Statistical analysis was performed using one-way analysis of variance (ANOVA). Results: Expression of α2-macroglobulin mRNA was lower in CO2 groups compared to the control group (p <0.05 at time 120 and 270). β-Fibrinogen message was diminished in CO2 0 and 120 groups compared to control. Serum levels of IL-6 and expression of metallothionein mRNA did not show significant differences between groups. Conclusions: These findings suggest that CO2 pneumoperitoneum downregulates the inflammatory response to LPS challenge. Start time of CO2 insufflation does not appear to alter hepatic expression of acute phase genes. The mechanism of α2-macroglobulin downregulation does not appear to be due to IL-6.

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