The effect of thrombin induced aggregation on human platelet glycosphingolipids

Subroto B Chatterjee, Charles C. Sweeley

Research output: Contribution to journalArticle

Abstract

Analyses were made of the glycosphingolipid composition of human platelets before and after treatment with thrombin. Within 10 min after incubation was initiated, there was approximately a two-fold increase in the concentration of hematoside. Concomitantly, the level of lactosylceramide was decreased about three-fold, and there was also a slight diminution in the ceramide levels of these cells. Platelets treated with phenylmethyl-sulfonyl fluoride and thrombin did not exhibit such an effect. These results suggest that lactosylceramide and ceramide are utilized as precursors in the biosynthesis of hematoside and that this conversion accompanies the aggregation of human platelets.

Original languageEnglish (US)
Pages (from-to)1310-1316
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume53
Issue number4
DOIs
StatePublished - Aug 21 1973
Externally publishedYes

Fingerprint

G(M3) Ganglioside
Glycosphingolipids
Ceramides
Platelets
Thrombin
Blood Platelets
Agglomeration
Platelet Aggregation
Biosynthesis
Chemical analysis
CDw17 antigen

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The effect of thrombin induced aggregation on human platelet glycosphingolipids. / Chatterjee, Subroto B; Sweeley, Charles C.

In: Biochemical and Biophysical Research Communications, Vol. 53, No. 4, 21.08.1973, p. 1310-1316.

Research output: Contribution to journalArticle

@article{249aaac3849244d4980e810ef8575e77,
title = "The effect of thrombin induced aggregation on human platelet glycosphingolipids",
abstract = "Analyses were made of the glycosphingolipid composition of human platelets before and after treatment with thrombin. Within 10 min after incubation was initiated, there was approximately a two-fold increase in the concentration of hematoside. Concomitantly, the level of lactosylceramide was decreased about three-fold, and there was also a slight diminution in the ceramide levels of these cells. Platelets treated with phenylmethyl-sulfonyl fluoride and thrombin did not exhibit such an effect. These results suggest that lactosylceramide and ceramide are utilized as precursors in the biosynthesis of hematoside and that this conversion accompanies the aggregation of human platelets.",
author = "Chatterjee, {Subroto B} and Sweeley, {Charles C.}",
year = "1973",
month = "8",
day = "21",
doi = "10.1016/0006-291X(73)90608-6",
language = "English (US)",
volume = "53",
pages = "1310--1316",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - The effect of thrombin induced aggregation on human platelet glycosphingolipids

AU - Chatterjee, Subroto B

AU - Sweeley, Charles C.

PY - 1973/8/21

Y1 - 1973/8/21

N2 - Analyses were made of the glycosphingolipid composition of human platelets before and after treatment with thrombin. Within 10 min after incubation was initiated, there was approximately a two-fold increase in the concentration of hematoside. Concomitantly, the level of lactosylceramide was decreased about three-fold, and there was also a slight diminution in the ceramide levels of these cells. Platelets treated with phenylmethyl-sulfonyl fluoride and thrombin did not exhibit such an effect. These results suggest that lactosylceramide and ceramide are utilized as precursors in the biosynthesis of hematoside and that this conversion accompanies the aggregation of human platelets.

AB - Analyses were made of the glycosphingolipid composition of human platelets before and after treatment with thrombin. Within 10 min after incubation was initiated, there was approximately a two-fold increase in the concentration of hematoside. Concomitantly, the level of lactosylceramide was decreased about three-fold, and there was also a slight diminution in the ceramide levels of these cells. Platelets treated with phenylmethyl-sulfonyl fluoride and thrombin did not exhibit such an effect. These results suggest that lactosylceramide and ceramide are utilized as precursors in the biosynthesis of hematoside and that this conversion accompanies the aggregation of human platelets.

UR - http://www.scopus.com/inward/record.url?scp=0015801872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015801872&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(73)90608-6

DO - 10.1016/0006-291X(73)90608-6

M3 - Article

C2 - 4748822

AN - SCOPUS:0015801872

VL - 53

SP - 1310

EP - 1316

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -