The effect of thrombin induced aggregation on human platelet glycosphingolipids

Subroto Chatterjee, Charles C. Sweeley

Research output: Contribution to journalArticlepeer-review

Abstract

Analyses were made of the glycosphingolipid composition of human platelets before and after treatment with thrombin. Within 10 min after incubation was initiated, there was approximately a two-fold increase in the concentration of hematoside. Concomitantly, the level of lactosylceramide was decreased about three-fold, and there was also a slight diminution in the ceramide levels of these cells. Platelets treated with phenylmethyl-sulfonyl fluoride and thrombin did not exhibit such an effect. These results suggest that lactosylceramide and ceramide are utilized as precursors in the biosynthesis of hematoside and that this conversion accompanies the aggregation of human platelets.

Original languageEnglish (US)
Pages (from-to)1310-1316
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume53
Issue number4
DOIs
StatePublished - Aug 21 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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