The effect of three serum basic proteins on the mass of lipids in normal and hyperapoB fibroblasts

Peter O. Kwiterovich, Mahnaz Motevalli, Michael Miller

Research output: Contribution to journalArticlepeer-review

Abstract

We studied whether serum basic proteins (BPs) produce abnormal changes in the mass of cellular lipids in fibroblasts from patients with hyperapobetalipoproteinemia (hyperapoB) and if inhibition or stimulation of protein kinase C affects these processes. In normal cells, BP I increased the mean mass of triglycerides about twofold, whereas there was significantly less stimulation in hyperapoB cells (P<.005). The increase in the mass of cell cholesteryl esters seen in normal cells with BP I was also significantly reduced in hyperapoB cells (P<.005). In contrast, BP II abnormally stimulated the mass of cell cholesteryl esters sixfold in hyperapoB cells (P<.005). BP I also stimulated the mass of total phospholipids about twofold in normal cells, an effect that was reduced by about one third in hyperapoB cells (P=.08). No abnormality was found in hyperapoB cells with BP III. H-7, an inhibitor of protein kinase C, decreased the effects of BP I and BP II in normal and hyperapoB cells. C:8, an analogue of diacylglycerol, activated protein kinase C and stimulated triglyceride formation in normal (fourfold) and hyperapoB (fivefold) cells in the absence of BP I. When added with C:8, BP I further increased triglyceride production 1.5-fold in normal cells but not in hyperapoB cells. Two cellular abnormalities in lipid metabolism in hyperapoB fibroblasts were found, one with BP I, another with BP II. Protein kinase C activity was not deficient in hyperapoB cells, and the defect(s) may occur at another, perhaps earlier, step in the pathway.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Volume14
Issue number1
DOIs
StatePublished - 1994

Keywords

  • Cholesterol
  • Cholesteryl esters
  • Free fatty acids
  • Hyperlipidemia
  • Phospholipids
  • Protein kinase C
  • Triglycerides

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'The effect of three serum basic proteins on the mass of lipids in normal and hyperapoB fibroblasts'. Together they form a unique fingerprint.

Cite this