The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

Sing Yian Chew, Ruifa Mi, Ahmet Hoke, Kam W. Leong

Research output: Contribution to journalArticlepeer-review

Abstract

Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Büngner prior to implantation. Aligned electrospun poly(ε-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Büngner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on two-dimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, myelin-associated glycoprotein (MAG), and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly oriented PCL electrospun fibers. However, up-regulation of the myelin-specific gene, P0, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation.

Original languageEnglish (US)
Pages (from-to)653-661
Number of pages9
JournalBiomaterials
Volume29
Issue number6
DOIs
StatePublished - Feb 2008

Keywords

  • Contact guidance
  • Electrospinning
  • Neural tissue engineering
  • Peripheral nerve regeneration
  • Schwann cell

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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