TY - JOUR
T1 - The effect of pantethine and ultraviolet-B radiation on the development of lenticular opacity in the emory mouse
AU - Congdon, Nathan
AU - West, Sheila
AU - Duncan, Donald
AU - Fisher, Daniel
AU - Vitale, Susan
AU - Rieger, Kenneth
AU - Urist, John
AU - Hazelwood, David
AU - Sanchez, Ana
AU - Pham, T.
AU - Cole, L.
AU - McNaughton, C.
N1 - Funding Information:
This study was supported in part by NIH Grant EY01765 (Wilmer Biostatistics Center) and from the Association for Aging Research (AFAR). Dr. Congdon was supported in part by the Heed Foundation, Cleveland OH. Dr. West is a Research to Prevent Blindness Senior Scientific Investigator
PY - 2000/1
Y1 - 2000/1
N2 - Purpose. Few studies have examined the impact of long-term treatments or exposures on the development of cataract in maturity-onset animal models. We studied the effect of treatment with D-pantethine and exposure to ultraviolet-B (UVB) radiation on the development of lenticular opacity in the Emory mouse. Methods. A total of 164 Emory mice were randomized by litter at weaning to exposure to UVB light at 12 mJ/cm2 for 6 hr/day (UV) or usual room light (A), and within litter, were further randomized to bi-weekly intra-peritoneal injections of 0.8 g/kg pantethine (T) or no treatment (C). Retro illumination lens photos were taken at 2, 4, 6, 8, and 10 months after weaning, and graded in masked fashion. The animals were sacrificed at 10 months and the lenses analyzed for total pantethine and total cysteamine. Results. Lens pantethine and cysteamine levels were significantly (P < 0.001) higher for the T as compared to C litters. Mean cataract grade increased monotonically over time for all four groups. Unadjusted mean grade for the AT group at 8 (1.32) and 10 (1.86) months appeared lower than for the other groups (AC: 2.17, 2.39; UVC: 1.77, 2.40; UVT: 1.88, 2.37). However, the mean grade for the pantethine-treated litters did not differ significantly from the untreated litters except at 2 months (when untreated litters had significantly lower grades), when adjusting for UV treatment, gender and litter effect. No significant difference in cataract score existed between UV-exposed and ambient litters. Mortality was higher among pantethine-treated (hazard ratio = 1.8, p = 0.05) and UV-exposed animals (hazard ratio = 1.8, p = 0.03) than among the untreated and unexposed litters. Conclusion. Significantly increased lens levels of pantethine are achieved with long-term intra-peritoneal dosing. The impact of pantethine on the progression of lenticular opacity in the Emory mouse is less than has been reported in other models. This level of chronic UVB exposure appeared to have no effect on the development of cataract in this model.
AB - Purpose. Few studies have examined the impact of long-term treatments or exposures on the development of cataract in maturity-onset animal models. We studied the effect of treatment with D-pantethine and exposure to ultraviolet-B (UVB) radiation on the development of lenticular opacity in the Emory mouse. Methods. A total of 164 Emory mice were randomized by litter at weaning to exposure to UVB light at 12 mJ/cm2 for 6 hr/day (UV) or usual room light (A), and within litter, were further randomized to bi-weekly intra-peritoneal injections of 0.8 g/kg pantethine (T) or no treatment (C). Retro illumination lens photos were taken at 2, 4, 6, 8, and 10 months after weaning, and graded in masked fashion. The animals were sacrificed at 10 months and the lenses analyzed for total pantethine and total cysteamine. Results. Lens pantethine and cysteamine levels were significantly (P < 0.001) higher for the T as compared to C litters. Mean cataract grade increased monotonically over time for all four groups. Unadjusted mean grade for the AT group at 8 (1.32) and 10 (1.86) months appeared lower than for the other groups (AC: 2.17, 2.39; UVC: 1.77, 2.40; UVT: 1.88, 2.37). However, the mean grade for the pantethine-treated litters did not differ significantly from the untreated litters except at 2 months (when untreated litters had significantly lower grades), when adjusting for UV treatment, gender and litter effect. No significant difference in cataract score existed between UV-exposed and ambient litters. Mortality was higher among pantethine-treated (hazard ratio = 1.8, p = 0.05) and UV-exposed animals (hazard ratio = 1.8, p = 0.03) than among the untreated and unexposed litters. Conclusion. Significantly increased lens levels of pantethine are achieved with long-term intra-peritoneal dosing. The impact of pantethine on the progression of lenticular opacity in the Emory mouse is less than has been reported in other models. This level of chronic UVB exposure appeared to have no effect on the development of cataract in this model.
KW - Cataract
KW - Emory mouse
KW - Lens
KW - Pantethine
KW - UV light
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U2 - 10.1076/0271-3683(200001)2011-HFT017
DO - 10.1076/0271-3683(200001)2011-HFT017
M3 - Article
C2 - 10611711
AN - SCOPUS:6744248641
SN - 0271-3683
VL - 20
SP - 17
EP - 24
JO - Current Eye Research
JF - Current Eye Research
IS - 1
ER -