The Effect of Negative Pressure Wound Therapy with Antiseptic Instillation on Biofilm Formation in a Porcine Model of Infected Spinal Instrumentation

Devinder P. Singh, Arvind U. Gowda, Karan Chopra, Michael Tholen, Sarah Chang, Vasilios Mavrophilipos, Nina Semsarzadeh, Yvonne Rasko, Luther Holton

Research output: Contribution to journalArticlepeer-review

Abstract

Objective. This study evaluates the effect of negative pressure wound therapy with antiseptic instillation (NPWTi) in the clearance of infection and biofilm formation in an in vivo model of infected spinal implants compared to traditional treatment modalities. Materials and Methods. Five pigs underwent titanium rod implantation of their spinous processes followed by injection of 1 x 106 CFUs/100μL of methicillinresistant Staphylococcus aureus through the fascia at each site. At 1 week postoperatively, an experimental arm of 3 pigs received NPWTi, and a control arm of 2 pigs received wet-to-dry dressings. The persistence of local infection in the experimental group was compared to the control group using tissue cultures. Biofilm development on spinal implants was evaluated using scanning electron microscopy. Results. Mean bacterial count showed a statistical difference between the experimental and the control groups (P < .05). Scanning electron microscopy revealed the presence of uniform biofilm formation across the surface of control group instrumentation, whereas the experimental group showed interrupted areas between biofilm formations. Conclusion. The authors concluded that NPWTi is associated with decreased bacterial load and biofilm formation compared to wet-to-dry dressings in an in vivo porcine model of infected spinal instrumentation.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalWounds
Volume29
Issue number6
StatePublished - Jun 2017

Keywords

  • biofilm
  • negative pressure wound therapy
  • spinal infection secondary spinal surgery

ASJC Scopus subject areas

  • Surgery
  • Medical–Surgical

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