The effect of nanofiber-guided cell alignment on the preferential differentiation of neural stem cells

Shawn H. Lim, Xingyu Y. Liu, Hongjun Song, Kevin J. Yarema, Hai Quan Mao

Research output: Contribution to journalArticle

Abstract

Stem cells display sensitivity to substrate presentation of topographical cues via changes in cell morphology. These biomechanical responses may be transmitted to the nucleus through cytoskeletal-linked signaling pathways. Here we investigate the influence of aligned substratum topography on the cell morphology and subsequently, the neuronal differentiation capabilities of adult neural stem cells (ANSCs). ANSCs that were cultured on aligned fibers elongated along the major fiber axis. Upon induction of differentiation with retinoic acid, a higher fraction of cells on aligned fibers exhibited markers of neuronal differentiation as compared with cells on random fiber or unpatterned surfaces. This effect was in part due to substrate selectivity, whereby aligned fiber substrates were less receptive to the attachment and continued survival of oligodendrocytes than random fiber or unpatterned substrates. Substrate-induced elongation alone was also effective in upregulating canonical Wnt signaling in ANSCs, which was further potentiated by retinoic acid treatment. These findings suggest a mechanism by which morphological control of stem cells operates in concert with biochemical cues for cell fate determination.

Original languageEnglish (US)
Pages (from-to)9031-9039
Number of pages9
JournalBiomaterials
Volume31
Issue number34
DOIs
StatePublished - Dec 1 2010

Keywords

  • Aligned nanofiber
  • Differentiation
  • Electrospun fiber
  • Neural stem cell

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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