TY - JOUR
T1 - The effect of intermittent pneumatic compression of legs on the levels of nitric oxide related species in blood and on arterial function in the arm
AU - Rifkind, Joseph M.
AU - Nagababu, Enika
AU - Dobrosielski, Devon A.
AU - Salgado, Maria T.
AU - Lima, Michael
AU - Ouyang, Pamela
AU - Silber, Harry A.
N1 - Funding Information:
The authors gratefully acknowledge Sandra Lima for acquiring the ultrasound images, and ACI Medical for providing the ArtAssist Intermittent Pneumatic Compression devices. This research was supported in part by the Johns Hopkins Cardiology Division and by the Intramural Research Program of the NIH , National Institutes of Aging . This work was also made possible by Grant Number UL1 RR 025005 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH), and NIH Roadmap for Medical Research . The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official view of NCRR or NIH. Information on NCRR is available at http://www.ncrr.nih.gov/ . Information on Re-engineering the Clinical Research Enterprise can be obtained from http://nihroadmap.nih.gov/clinical research/overview-translational.asp.
PY - 2014/8/31
Y1 - 2014/8/31
N2 - Background Intermittent pneumatic compression (IPC) of legs exerts beneficial local vascular effects, possibly through local release of nitric oxide (NO). However, studies demonstrating systemic transport of nitrogen oxide species and release of NO prompt the question of whether IPC could also exert nonlocal effects. We tested whether IPC (1) affects systemic levels of nitrite, S-nitrosothiols and red blood cell (RBC) NO, and (2) exerts vasoactive effects in the brachial artery (BA), although this hypothesis-generating pilot study did not investigate cause and effect relationship between (1) and (2). Methods In 10 healthy subjects, ages 24-39 years, we measured plasma nitrite, plasma S-nitrosothiols and RBC-NO from venous blood samples drawn before and after IPC treatment. We also measured BA responses to 5 min of upper arm occlusion at rest and during1 h of leg IPC. Results There was a significant decrease in plasma nitrite (112 ± 26 nM to 90 ± 15 nM, p = 0.0008) and RBC-NO (129 ± 72 nM to 102 ± 41 nM, p = 0.02). Plasma S-nitrosothiols were unchanged (5.79 ± 4.81 nM to 6.27 ± 5.79 nM, p = 0.3). BA occlusion-mediated constriction (OMC) was significantly attenuated with IPC treatment (-43 ± 13% to -33 ± 12%, p = 0.003). High-flow mediated BA dilation was unchanged (13.3 ± 9.4% to 11.5 ± 7.2%, p = 0.2). Conclusion Plasma nitrite, RBC-NO, and BA OMC decreased with leg IPC. We hypothesize that this decrease in circulatory pool of plasma nitrite and RBC-NO may result from the transfer of their NO-bioactivity from blood to the hypoxic arm tissue, to be stored and released under hypoxic stress and oppose OMC. Future studies should investigate whether IPC-induced decreases in brachial OMC are caused by the changes in systemic NO activity, and whether leg IPC could benefit distant arterial function in systemic cardiovascular disease.
AB - Background Intermittent pneumatic compression (IPC) of legs exerts beneficial local vascular effects, possibly through local release of nitric oxide (NO). However, studies demonstrating systemic transport of nitrogen oxide species and release of NO prompt the question of whether IPC could also exert nonlocal effects. We tested whether IPC (1) affects systemic levels of nitrite, S-nitrosothiols and red blood cell (RBC) NO, and (2) exerts vasoactive effects in the brachial artery (BA), although this hypothesis-generating pilot study did not investigate cause and effect relationship between (1) and (2). Methods In 10 healthy subjects, ages 24-39 years, we measured plasma nitrite, plasma S-nitrosothiols and RBC-NO from venous blood samples drawn before and after IPC treatment. We also measured BA responses to 5 min of upper arm occlusion at rest and during1 h of leg IPC. Results There was a significant decrease in plasma nitrite (112 ± 26 nM to 90 ± 15 nM, p = 0.0008) and RBC-NO (129 ± 72 nM to 102 ± 41 nM, p = 0.02). Plasma S-nitrosothiols were unchanged (5.79 ± 4.81 nM to 6.27 ± 5.79 nM, p = 0.3). BA occlusion-mediated constriction (OMC) was significantly attenuated with IPC treatment (-43 ± 13% to -33 ± 12%, p = 0.003). High-flow mediated BA dilation was unchanged (13.3 ± 9.4% to 11.5 ± 7.2%, p = 0.2). Conclusion Plasma nitrite, RBC-NO, and BA OMC decreased with leg IPC. We hypothesize that this decrease in circulatory pool of plasma nitrite and RBC-NO may result from the transfer of their NO-bioactivity from blood to the hypoxic arm tissue, to be stored and released under hypoxic stress and oppose OMC. Future studies should investigate whether IPC-induced decreases in brachial OMC are caused by the changes in systemic NO activity, and whether leg IPC could benefit distant arterial function in systemic cardiovascular disease.
KW - Flow mediated dilation
KW - Intermittent pneumatic compression
KW - Low-flow mediated constriction
KW - Nitric oxide
KW - Nitrite
KW - Occlusion-mediated constriction
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U2 - 10.1016/j.niox.2014.06.007
DO - 10.1016/j.niox.2014.06.007
M3 - Article
C2 - 24973574
AN - SCOPUS:84905246515
VL - 40
SP - 117
EP - 122
JO - Nitric Oxide - Biology and Chemistry
JF - Nitric Oxide - Biology and Chemistry
SN - 1089-8603
ER -