The effect of hemin in vitro and in vivo on human erythroid progenitor cells.

F. J. Kaye, R. S. Weinberg, J. M. Schofield, B. P. Alter

Research output: Contribution to journalArticle

Abstract

Hemin stimulates erythropoiesis and hemoglobin synthesis in vitro. We cultured erythroid progenitor cells from normal individuals, patients with sickle cell anemia, and a patient with acute variegate porphyria who received intravenous hemin treatment, with 0-800 microM hemin added in vitro. Fifty to 200 microM hemin consistently stimulated colony growth from normal donors 2- to 8-fold, while concentrations of up to 400 microM were stimulatory in cultures from donors with sickle cell anemia. In vivo hemin decreased the number of blood BFU-e in the patient with porphyria, but did not abrogate the in vitro stimulatory effect of hemin. Hemin concentrations which increased colony numbers increased gamma globin synthesis in some studies and decreased it in others. Hemin thus has clearcut erythroid growth-potentiating activity, although a consistent effect on globin chain regulation is not apparent.

Original languageEnglish (US)
Pages (from-to)74-88
Number of pages15
JournalInternational Journal of Cell Cloning
Volume5
Issue number1
StatePublished - Jan 1987
Externally publishedYes

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Hemin
Erythroid Precursor Cells
Sickle Cell Anemia
Variegate Porphyria
Tissue Donors
gamma-Globins
Acute Intermittent Porphyria
Porphyrias
Globins
In Vitro Techniques
Erythropoiesis
Growth
Cultured Cells
Hemoglobins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kaye, F. J., Weinberg, R. S., Schofield, J. M., & Alter, B. P. (1987). The effect of hemin in vitro and in vivo on human erythroid progenitor cells. International Journal of Cell Cloning, 5(1), 74-88.

The effect of hemin in vitro and in vivo on human erythroid progenitor cells. / Kaye, F. J.; Weinberg, R. S.; Schofield, J. M.; Alter, B. P.

In: International Journal of Cell Cloning, Vol. 5, No. 1, 01.1987, p. 74-88.

Research output: Contribution to journalArticle

Kaye, FJ, Weinberg, RS, Schofield, JM & Alter, BP 1987, 'The effect of hemin in vitro and in vivo on human erythroid progenitor cells.', International Journal of Cell Cloning, vol. 5, no. 1, pp. 74-88.
Kaye, F. J. ; Weinberg, R. S. ; Schofield, J. M. ; Alter, B. P. / The effect of hemin in vitro and in vivo on human erythroid progenitor cells. In: International Journal of Cell Cloning. 1987 ; Vol. 5, No. 1. pp. 74-88.
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