The effect of graphene oxide on conformation change, aggregation and cytotoxicity of HIV-1 regulatory protein (Vpr)

Min Zhang, Xiaobo Mao, Chenxuan Wang, Wenfeng Zeng, Chunling Zhang, Zhongjun Li, Ying Fang, Yanlian Yang, Wei Liang, Chen Wang

Research output: Contribution to journalArticle

Abstract

The fragment of viral protein R (Vpr), Vpr13-33, plays an important role in regulating nuclear importing of HIV through ion channel formation with a leucine-zipper-like α-helical conformation. Herein we report an approach to reduce cytotoxicity of Vpr13-33 by graphene oxide induced conformation change and aggregation. Preferential adsorption of Vpr13-33 on graphene oxide accompanied by conformation change from α-helix to β-sheet structures has been observed by using atomic force microscopy (AFM) and circular dichroism (CD). The submolecular structures of the Vpr13-33 peptide assembly on graphite surface have been identified by using scanning tunneling microscopy (STM), which confirms the β-sheet structures of Vpr13-33 on graphene oxide surface. The reduced cytotoxicity of Vpr13-33 to neuroblastoma cells and T cells are detected by MTT assay, which could be associated with the conformation change and stimulated aggregation of Vpr13-33 upon addition of graphene oxide through hydrophobic interaction. Furthermore, fluorescent leakage assay by using large unilamellar vesicles (LUVs) indicated that the GO reduced Vpr13-33-induced cytotoxicity could be associated with the inhibited " pore forming" function of Vpr13-33 by conformation change and aggregation.

Original languageEnglish (US)
Pages (from-to)1383-1390
Number of pages8
JournalBiomaterials
Volume34
Issue number4
DOIs
StatePublished - Jan 2013
Externally publishedYes

Fingerprint

vpr Gene Products
Graphite
Cytotoxicity
Oxides
Graphene
Conformations
HIV-1
Agglomeration
Proteins
Assays
Scanning Tunnelling Microscopy
Leucine Zippers
Unilamellar Liposomes
T-cells
Atomic Force Microscopy
Fasteners
Dichroism
Scanning tunneling microscopy
Circular Dichroism
Neuroblastoma

Keywords

  • Aggregation
  • Conformation change
  • Cytotoxicity
  • Graphene oxide
  • Hydrophobic interaction
  • Vpr13-33

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

The effect of graphene oxide on conformation change, aggregation and cytotoxicity of HIV-1 regulatory protein (Vpr). / Zhang, Min; Mao, Xiaobo; Wang, Chenxuan; Zeng, Wenfeng; Zhang, Chunling; Li, Zhongjun; Fang, Ying; Yang, Yanlian; Liang, Wei; Wang, Chen.

In: Biomaterials, Vol. 34, No. 4, 01.2013, p. 1383-1390.

Research output: Contribution to journalArticle

Zhang, M, Mao, X, Wang, C, Zeng, W, Zhang, C, Li, Z, Fang, Y, Yang, Y, Liang, W & Wang, C 2013, 'The effect of graphene oxide on conformation change, aggregation and cytotoxicity of HIV-1 regulatory protein (Vpr)', Biomaterials, vol. 34, no. 4, pp. 1383-1390. https://doi.org/10.1016/j.biomaterials.2012.10.067
Zhang, Min ; Mao, Xiaobo ; Wang, Chenxuan ; Zeng, Wenfeng ; Zhang, Chunling ; Li, Zhongjun ; Fang, Ying ; Yang, Yanlian ; Liang, Wei ; Wang, Chen. / The effect of graphene oxide on conformation change, aggregation and cytotoxicity of HIV-1 regulatory protein (Vpr). In: Biomaterials. 2013 ; Vol. 34, No. 4. pp. 1383-1390.
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