The effect of extracellular matrix interactions on morphologic transformation in vitro

Kenneth J. Pienta, Brian C. Murphy, Robert H. Getzenberg, Donald S. Coffey

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


There is emerging evidence that the structure and function of a cell is dependent in part on the contacts that cells make with the extracellular matrix. We report here the effect of extracellular matrices secreted from both normal and tumor cells have on the structure of normal rat kidney epithelial cells. Normal rat kidney cells plated on the basement membrane secreted by tumor cells adopt a morphology and phenotype which closely resembles a Kirsten-ras transformed normal rat kidney cell. This morphologic transformation was not observed for cells plated on individual extracellular matrix components or on basement membrane secreted by normal placenta cells. This suggests that tumor derived basement membrane has unique characteristics which may cause morphologic transformation of normal rat kidney cells.

Original languageEnglish (US)
Pages (from-to)333-339
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Aug 30 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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