Abstract
Concanavalin A (con A), a phytohemagglutinin, weakly aggregated washed platelets but inhibited their aggregation by thrombin. Concanavalin A and thrombin both released serotonin, nucleotides, and α-mannosidase from platelets. At suboptimal concentrations of con A and thrombin, a cumulative effect upon release of these substances was often observed. Glycosidases other than α-mannosidase (β-glucuronidase, N-acetyl-galactosaminidase, N-acetyl-glucosaminidase, and β-galactosidase) were minimally released from platelets by con A, and their release by thrombin was inhibited. Serotonin released initially by con A was re-accumulated by platelets within 30 minutes; however, thereafter these cells did not release serotonin after exposure to thrombin. The results suggest competition between con A and thrombin for receptor sites on the platelet membrane and suggest the possibility of more than one kind of receptor for thrombin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 887-895 |
| Number of pages | 9 |
| Journal | The Journal of laboratory and clinical medicine |
| Volume | 83 |
| Issue number | 6 |
| State | Published - Jun 1974 |
| Externally published | Yes |
ASJC Scopus subject areas
- Pathology and Forensic Medicine