The effect of cobalt chloride on skin flap survival

A. Atabey, M. J. Im, F. M. Akgur, Craig Vander Kolk, Paul Manson

Research output: Contribution to journalArticle

Abstract

The effects of cobalt chloride on ischaemia-reperfusion injury were evaluated in skin flaps. Groin neurovascular island flaps, 3 x 6 cm, were elevated in rats and subjected to primary and secondary ischaemia. Primary ischaemia was produced by 1 hour occlusion of the femoral artery and vein and, 22 hours later, secondary ischaemia was produced by 3 hours venous occlusion. The treatment group received intraperitoneal cobalt chloride (5 or 10 mg/kg) at the time of secondary ischaemia. Flap survival was 80% in the control, 10 to 20% in the cobalt chloride treated, and 20% in the cobalt plus mannitol group. Mannitol (100 mg/kg) failed to prevent the harmful effects of cobalt. Skin flaps exposed to cobalt chloride exhibited increased thiobarbituric acid reactant (TEAR) levels of 20 to 30 times normal. Of the antioxidant enzymes, glutathione peroxidase activity increased by 40% (P <0.01), whereas glutathione reductase activity decreased by 40% (P <0.01) in the cobalt exposed groups. Glucose 6-phosphate dehydrogenase activity was not affected.

Original languageEnglish (US)
Pages (from-to)321-324
Number of pages4
JournalBritish Journal of Plastic Surgery
Volume49
Issue number5
DOIs
StatePublished - Jul 1996

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Ischemia
Cobalt
Skin
Mannitol
Surgical Flaps
Femoral Vein
Glutathione Reductase
Groin
Glucosephosphate Dehydrogenase
Femoral Artery
Glutathione Peroxidase
Reperfusion Injury
Antioxidants
cobaltous chloride
Enzymes

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

The effect of cobalt chloride on skin flap survival. / Atabey, A.; Im, M. J.; Akgur, F. M.; Vander Kolk, Craig; Manson, Paul.

In: British Journal of Plastic Surgery, Vol. 49, No. 5, 07.1996, p. 321-324.

Research output: Contribution to journalArticle

Atabey, A. ; Im, M. J. ; Akgur, F. M. ; Vander Kolk, Craig ; Manson, Paul. / The effect of cobalt chloride on skin flap survival. In: British Journal of Plastic Surgery. 1996 ; Vol. 49, No. 5. pp. 321-324.
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