The effect of chronic vitamin deficiency and long term very low dose exposure to 6 pesticides mixture on neurological outcomes – A real-life risk simulation approach

Aristidis Tsatsakis, Nadezhda V. Tyshko, Anca Oana Docea, Svetlana I. Shestakova, Yuliia S. Sidorova, Nikita A. Petrov, Ovidiu Zlatian, Mojmir Mach, Thomas Hartung, Victor A. Tutelyan

Research output: Contribution to journalArticle

Abstract

We investigate the long-term effect of very-low dose exposure to a mixture of six pesticides associated with hydrophilic vitamin deficiency on the neurobehavioral outcomes of rats. Two hundred Wistar rats were divided into four groups, two control groups, a vitamin sufficient control group and a vitamin deficiency control group and 2 test groups, a vitamin sufficient test group, and a vitamin deficiency group. The test groups were exposed for 9 months to a mixture of diquat, imazamox, imazethapyr, tepraloxydin, bentazone and acifluorfen in doses of 0.01xNOAEL (mg/kg bw/day). After 9 months of exposure, the behavior changes were evaluated by open field test and elevated plus maze test and the memory was assessed by passive avoidance test. Chronic vitamin deficiency decreased locomotor and special orientation activity and increased anxiety-like behavior in rats. Exposure to very low doses of a mixture of 6 pesticides caused central nervous effects, manifested as decreased locomotor activity, and increased anxiety levels. Vitamin deficiency and low dose chronic pesticides mixture exposure thus affected the central nervous system, especially long-term memory.

Original languageEnglish (US)
Pages (from-to)96-106
Number of pages11
JournalToxicology Letters
Volume315
DOIs
StatePublished - Oct 15 2019

Keywords

  • Elevated plus maze test
  • Hormesis
  • Mixture
  • Open field test
  • Passive avoidance test
  • Pesticides
  • Real-life risk simulation
  • Vitamin deficiency

ASJC Scopus subject areas

  • Toxicology

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