The effect of carbon dioxide pneumoperitoneum on the healing colonic anastomosis in rats

Leonardo de Castro Durães, Igor Eduardo Caetano Farias, Pedro Henrique Alves de Morais, Paulo Gonçalves de Oliveira, Florêncio Figueiredo Cavalcanti Neto, Ruy Souza Lino Junior, Omar Feres, João Batista de Sousa

Research output: Contribution to journalArticlepeer-review

Abstract

PURPOSE: To investigate the effects of pneumoperitoneum on colonic anastomosis healing. METHODS: Colonic anastomosis was performed in 120 rats divided into four groups: Group I - pneumoperitoneum before laparotomy, Group II - pneumoperitoneum after laparorrhaphy, Group III - pneumoperitoneum before laparotomy and after laparorrhaphy, Group IV - no pneumoperitoneum (control group). Pneumoperitoneum pressure was 5 mmHg. Animals were killed on the 3rd, 7th and 14th postoperative day. Hhistopathological features, anastomosis breaking strength, collagen histomorphometry and hydroxyproline concentration were assessed. RESULTS: Breaking strength between groups: (day 3, p = 0.165; day 7, p = 0.219; day 14, p = 0.539). Histopathology revealed that group II had, on day 7, less infiltration of mononuclear cells (p = 0.006), greater infiltration of polymorphonuclear cells (p = 0.001) and greater necrosis (p = 0.001); and on day 14, less fibrosis. Histomorphometry revealed a decrease in collagen in groups I and III (p < 0.001) on day 7 and an increase in groups I and II on day 14 (p < 0.001). Hydroxyproline concentration was similar for groups on days 3 (p = 0.152), 7 (p = 0.913) or 14 (p = 0.981). CONCLUSION: Carbon dioxide does not impair the healing of colonic anastomosis in rats.

Original languageEnglish (US)
Pages (from-to)670-677
Number of pages8
JournalActa cirurgica brasileira
Volume28
Issue number9
DOIs
StatePublished - Sep 2013

Keywords

  • Anastomosis
  • Artificial
  • Carbon dioxide
  • Colon
  • Pneumoperitoneum
  • Rats
  • Surgical
  • Wound healing

ASJC Scopus subject areas

  • Surgery

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